中国医学科学院学报

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中国医学科学院学报

中国医学科学院学报 ›› 2019, Vol. 41 ›› Issue (3): 307-314.doi: 10.3881/j.issn.1000-503X.10370

• 论著 • 上一篇    下一篇

枯草芽孢杆菌菌株CGMCC 12426的全基因组测序与分析

董辉,李轩豪,常静林,贺欣,侯沁莲,龙伟()   

  1. 中国医学科学院 北京协和医学院 放射医学研究所天津市放射医学与分子核医学重点实验室,天津 300192
  • 收稿日期:2018-03-05 出版日期:2019-06-30 发布日期:2019-07-04
  • 通讯作者: 龙伟 E-mail:longway@irm-cams.ac.cn
  • 基金资助:
    国家自然科学基金(81673160);国家自然科学基金(31300601);中国医学科学院医学与健康科技创新工程项目(2017-I2M-1-102);中国医学科学院医学与健康科技创新工程项目(2017-I2M-3-019)

Whole Genome Sequence Determination and Analysis of Bacillus Subtilis Strain CGMCC 12426

DONG Hui,LI Xuanhao,CHANG Jinglin,HE Xin,HOU Qinlian,LONG Wei()   

  1. Key Laboratory of Tianjin Radiation and Molecular Nuclear Medicine,Institute of Radiation Medicine,CAMS and PUMC,Tianjin 300192,China
  • Received:2018-03-05 Online:2019-06-30 Published:2019-07-04
  • Contact: Wei LONG E-mail:longway@irm-cams.ac.cn
  • Supported by:
    Supported by the National Natural Sciences Foundation of China(81673160);Supported by the National Natural Sciences Foundation of China(31300601);The CAMS Innovation Fund for Medical Sciences(2017-I2M-1-102);The CAMS Innovation Fund for Medical Sciences(2017-I2M-3-019)

摘要:

目的 描述枯草芽孢杆菌CGMCC 12426的微生物特征,对其进行全基因组测序和注释。方法 枯草芽孢杆菌菌株CGMCC 12426基因组DNA测序由太平洋生物技术公司的RS Ⅱ测序仪基于单分子实时测序(SMRT)技术平台完成,其注释是在NCBI原核基因组注释管道(pGAP)完成。结果 已发布的枯草芽孢杆菌CGMCC 12426的完整基因组序列是由1个环形的4 138 265 bp的染色体与1个74 165 bp的质粒组成,最终预测得到的4581个基因包括4222个编码序列、87个tRNA和30个rRNA(5S rRNA、16S rRNA和23S rRNA)序列。结论 为进一步阐明该菌株的遗传背景提供了基础,也为研究代谢和调控机制提供了重要机会。

关键词: 枯草芽孢杆菌, 基因组测序, 高效聚γ谷氨酸

Abstract:

Objective To describe the microbiological characteristics of Bacillus subtilis(B. subtilis)CGMCC 12426 and determine and analyze its complete genome sequences.Methods B. subtilis strain CGMCC 12426 genomic DNA sequencing was performed on a single molecule real-time sequencing(SMRT)platform and the annotation was completed in the NCBI Prokaryotic Genomic Annotation Pipeline(pGAP).Results The complete genomic sequences of the released B. subtilis CGMCC 12426 consisted of a 4 138 265-bp circular chromosome and a 74 165-bp plasmid,which resulted in the prediction of 4581 genes including 4222 coding sequences,87 tRNAs,and 30 rRNAs(which included 5S rRNA,16S rRNA,and 23S rRNA).Conclusion The genome sequencing provided a basis for further investigations on the genetic background of B. subtilis and on the metabolic and regulatory mechanisms.

Key words: Bacillus subtilis, genome sequence, poly-γ-glutamate

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