中国医学科学院学报

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中国医学科学院学报

中国医学科学院学报 ›› 2019, Vol. 41 ›› Issue (3): 300-306.doi: 10.3881/j.issn.1000-503X.10599

• 论著 • 上一篇    下一篇

不同炎症因子对人牙周膜成纤维细胞促红细胞生成素肝细胞激酶受体和配体的影响

徐晓南,王梦琳,张丁()   

  1. 中国医学科学院 北京协和医学院 北京协和医院口腔科,北京 100730
  • 收稿日期:2018-06-04 出版日期:2019-06-30 发布日期:2019-07-04
  • 通讯作者: 张丁 E-mail:dingz77@sina.com
  • 基金资助:
    国家自然科学基金(31371389)

Effects of Different Inflammatory Factors on Hepatocyte Kinase Receptors and Ligands in Human Periodontal Ligament Fibroblasts

XU Xiaonan,WANG Menglin,ZHANG Ding()   

  1. Department of Stomatology,PUMC Hospital,CAMS and PUMC,Beijing 100730,China
  • Received:2018-06-04 Online:2019-06-30 Published:2019-07-04
  • Contact: Ding ZHANG E-mail:dingz77@sina.com
  • Supported by:
    Supported by the National Natural Sciences Foundation of China(31371389)

摘要:

目的 观察不同炎症因子对人牙周膜成纤维细胞(hPDLFs)促红细胞生成素肝细胞激酶受体(Eph)和ephrin配体的影响。方法 采用10 ng/ml肿瘤坏死因子α(TNF-α)或10 ng/ml白细胞介素1β(IL-1β)刺激人牙周膜成纤维细胞,分别在0、1、2、6、12、24 h时,检测hPDLFs Eph受体及ephrin 配体在mRNA 和蛋白水平的表达变化。结果 TNF-α和IL-1β均可引起hPDLFs Eph受体和ephrin配体发生时间依赖性变化。两组中ephrinA2表达量24 h内均呈明显上升趋势(P均<0.05),TNF-α组的ephrinA2 mRNA 1 h时即出现明显高表达,24 h时TNF-α组的表达量明显高于IL-1β组(P<0.05)。EphB4在短时间高表达后,呈时间依赖性下降趋势。结论 TNF-α和IL-1β均可引起hPDLFs EphB4和ephrinA2的表达变化,两者引起的表达变化趋势一致,但TNF-α的作用更显著。

关键词: 炎症因子, 人牙周膜成纤维细胞, 促红细胞生成素肝细胞激酶受体, ephrin

Abstract:

Objective To investigate the effects of different inflammatory factors on hepatocyte kinase receptor(Eph)and ligand(ephrin)in human periodontal ligament fibroblasts(hPDLFs).Methods hPDLFs were stimulated with either 10 ng/ml tumor necrosis factor-α(TNF-α)or 10 ng/ml interleukin(IL)-1β,and then the expressions of Eph and ephrin at both mRNA and protein levels were determined at 0,1,2,6,12,and 24 hours.Results The levels of Eph receptors and ephrin ligand changed in a time-dependent manner in human periodontal ligament fibroblasts after treatment with TNF-α or IL-1β. The expression of ephrinA2 significantly increased in both groups within 24 hours(all P<0.05). In the TNF-α group,the mRNA expression of ephrinA2 significantly increased at 1 h and was significant higher that in the IL-1β group at 24 h(P<0.05). EphB4 showed a time-dependent decline after a short period of high expression.Conclusions Both TNF-α and IL-1β can cause changes in the expressions of Eph receptors and ephrin ligands in hPDLFs. The changes induced by both are consistent,although the effect of TNF-α is more pronounced.

Key words: inflammatory factors, human periodontal ligament fibroblasts, erythropoietin producing hepatocyte kinase receptors, ephrin

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