中国医学科学院学报

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中国医学科学院学报

中国医学科学院学报 ›› 2021, Vol. 43 ›› Issue (4): 536-544.doi: 10.3881/j.issn.1000-503X.13966

• 论著 • 上一篇    下一篇

自然衰老小鼠肝脏与血清的非靶向代谢组学特征

柳江枫1,杨晔宏1,武乐2,杨俊涛1()   

  1. 1中国医学科学院 北京协和医学院 基础医学研究所医学分子生物学国家重点实验室,北京 100005
    2南开大学统计与数据科学学院,天津 300071
  • 收稿日期:2021-03-23 出版日期:2021-08-30 发布日期:2021-09-03
  • 通讯作者: 杨俊涛 E-mail:yangjt@pumc.edu.cn
  • 基金资助:
    中国医学科学院医学与健康科技创新工程(CIFMS2017-I2M-1-008);中国医学科学院医学与健康科技创新工程(CIFMS2019-I2M-1-004)

Untargeted Metabolomic Profiling of Liver and Serum in Mouse during Normal Aging

LIU Jiangfeng1,YANG Yehong1,WU Yue2,YANG Juntao1()   

  1. 1State Key Laboratory of Medical Molecular Biology,Institute of Basic Medical Sciences, CAMS and PUMC,Beijing 100005,China
    2School of Statistics and Data Science,Nankai University,Tianjin 300071,China
  • Received:2021-03-23 Published:2021-08-30 Online:2021-09-03
  • Contact: YANG Juntao E-mail:yangjt@pumc.edu.cn
  • Supported by:
    Chinese Academy of Medical Sciences Innovation Fund for Medical Sciences(CIFMS2017-I2M-1-008);Chinese Academy of Medical Sciences Innovation Fund for Medical Sciences(CIFMS2019-I2M-1-004)

摘要:

目的 检测自然衰老小鼠肝脏与血清的代谢组学改变情况。方法 取10只2月龄小鼠与10只18月龄小鼠,分离生理条件下的肝脏和血清做样本制备,采用液相色谱-串联质谱技术做代谢物鉴定与定量,对过滤后的高质量数据做整体分析、差异筛选和差异代谢物功能分析。结果 质谱负离子和正离子模式在小鼠肝脏分别鉴定到242和399个代谢物,在小鼠血清分别鉴定到265和230个代谢物,年轻与年老小鼠代谢组的整体区分较小。衰老肝脏鉴定的上调代谢物涉及核黄素代谢、糖代谢和花生四烯酸代谢等通路,下调代谢物涉及嘧啶代谢、嘌呤代谢、甘油磷脂代谢、谷胱甘肽代谢和烟酰胺代谢。血清中伴随衰老改变的代谢物与多种核酸代谢相关途径相关,如嘧啶代谢、嘌呤代谢、一碳单位池、氨糖及核糖代谢等途径。结论 衰老小鼠肝脏及血清可能存在核酸代谢通路紊乱,本研究为衰老相关机制研究及干预途径选择提供了数据支持。

关键词: 衰老, 小鼠, 肝脏, 血清, 非靶向代谢组学

Abstract:

Objective To obtain the metabolome profiles in liver and serum of mice during normal aging. Methods The liver and serum samples of ten 2-month-old mice and ten 18-month-old C57BL/6J mice under physiological conditions were collected.Metabolites were identified and quantified by liquid chromatography-tandem mass spectrometry.The overall assessment,differential screening,and functional analysis were performed with the filtered high-quality data. Results In the negative-ion mode and positive-ion mode,242 and 399 metabolites were identified in the liver and 265 and 230 in serum,respectively.The difference of metabolome between young and old mice was moderate.The upregulated metabolites identified in aging liver were related to the metabolism of riboflavin,glucose,and arachidonic acid,while the downregulated ones were associated with the metabolism of pyrimidine,purine,glycerophospholipid,glutathione,and nicotinamide.Altered metabolites in serum during aging were involved in a variety of nucleic acid metabolism-related pathways,such as pyrimidine metabolism,purine metabolism,one carbon pool by folate,and amino sugar and nucleotide sugar metabolism. Conclusions The metabolome profiles of mouse liver and serum both revealed dysregulated nucleic acid metabolism pathways during normal aging.This study provides metabolome data for further research on aging-associated mechanism and may support the discovery of intervention methods for aging.

Key words: aging, mouse, liver, serum, untargeted metabolomic profiling

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