中国医学科学院学报

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中国医学科学院学报

中国医学科学院学报 ›› 2009, Vol. 31 ›› Issue (4): 410-412.doi: 10.3881/j.issn.1000-503X.2009.04.005

• 论著 • 上一篇    下一篇

耻垢分枝杆菌疫苗对小鼠巨噬细胞产生一氧化氮的影响

徐苗; 陈保文;沈小兵;苏城;王国治   

  1. 中国药品生物制品检定所细菌一室,北京 100050
  • 收稿日期:2009-03-31 修回日期:1900-01-01 出版日期:2009-08-30 发布日期:2009-08-30
  • 通讯作者: 王国治

Effect of Mycobacterium Smegmatis Vaccine on the Level of Nitric Oxide Produced by Peritoneal Macrophages in Mice

XU Miao; CHEN Bao-wen; SHEN Xiao-bing; SU Cheng; WANG Guo-zhi   

  1. 1st Bacterial Vaccine Division,National Institute for the Control of Pharmaceutical and Biological Products, Beijing 100050, China
  • Received:2009-03-31 Revised:1900-01-01 Online:2009-08-30 Published:2009-08-30
  • Contact: WANG Guo-zhi

摘要: 摘要:目的 探讨耻垢分枝杆菌疫苗对小鼠腹腔巨噬细胞产生一氧化氮的影响。 方法将Balb/c小鼠随机分成生理盐水对照组和耻垢分枝杆菌疫苗低、中、高剂量组,分别注射生理盐水和不同剂量的耻垢分枝杆菌疫苗。取小鼠腹腔巨噬细胞体外培养,化学显色法测定培养上清中一氧化氮的含量。结果 生理盐水对照组和耻垢分枝杆菌疫苗低、中、高剂量组产生的一氧化氮分别为(3.50±3.11)、(16.63±6.47)、(13.97±6.20)和(7.55±2.26)ng/ml,不同剂量疫苗免疫小鼠产生的一氧化氮水平均显著高于对照组(P<0.01)。 结论耻垢分枝杆菌疫苗可促进小鼠巨噬细胞产生一氧化氮。

关键词: 耻垢分枝杆菌, 疫苗, 一氧化氮

Abstract: ABSTRACT:Objective To study the effect of Mycobacterium smegmatis vaccine on the level of nitric oxide (NO) produced by peritoneal macrophages in immunized mice. Methods Balb/c mice were randomized into low-dose, middle-dose, and high-dose groups (injected with different doses of Mycobacterium smegmatis vaccine) and a control group (injected with normal saline). Then the peritoneal macrophages were cultured with lipopolysaccharide in vitro. The supernatants were collected and the concentrations of NO were analyzed through the reaction with Griess reagents. Results The levels of NO produced by the peritoneal macrophages in the control group, low-dose group, middle-dose group, and high-dose group were (3.50±3.11) ,(16.63±6.47), (13.97±6.20),and (7.55±2.26) ng/ml, respectively. The levels of NO in all dosing groups were significantly different from that in control group (P<0.01). Conclusion Mycobacterium smegmatis vaccine can promote the peritoneal macrophages to produce NO in mice.

Key words: mycobacterium smecmatis, vaccine, nitric oxide