中国医学科学院学报

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中国医学科学院学报

中国医学科学院学报 ›› 2009, Vol. 31 ›› Issue (4): 413-416.doi: 10.3881/j.issn.1000-503X.2009.04.006

• 论著 • 上一篇    下一篇

噬菌体硝酸盐还原实验方法的建立与评估

邢爱英; 刘忠泉; 贾红彦; 古淑香; 张宗德   

  1. 北京市结核病胸部肿瘤研究所结核病分子生物学研究室,北京 101149
  • 收稿日期:2009-02-12 修回日期:1900-01-01 出版日期:2009-08-30 发布日期:2009-08-30
  • 通讯作者: 张宗德

Establishment and Evaluation of Nitrate Reductase Combined with Mycobacteriophage Assay

XING Ai-ying; LIU Zhong-quan; JIA Hong-yan; GU Shu-xiang;ZHANG Zong-de   

  1. Laboratory of Molecular Biology for Tuberculosis, Beijing Tuberculosis and Thoracic
    Tumor Research Institute, Beijing 101149,China
  • Received:2009-02-12 Revised:1900-01-01 Online:2009-08-30 Published:2009-08-30
  • Contact: ZHANG Zong-de

摘要: 摘要:目的 采用噬菌体与硝酸盐还原实验结合的技术,以期建立一种新的快速、简易、廉价的结核杆菌药敏检测方法。方法 对49株结核杆菌的临床分离株以噬菌体硝酸盐还原实验(PhaB-NRA法)与改良罗氏绝对浓度法对结核杆菌进行利福平、异烟肼药物敏感性测定,以绝对浓度法作为参照标准判定PhaB-NRA法的敏感性、特异性、准确性及其可行性。结果 PhaB-NRA法对利福平进行药物敏感性测定的敏感性、特异性、准确性分别为89.1%、91.67%、89.8%;对异烟肼进行药物敏感性测定的敏感性、特异性、准确性分别为86.21%、90.0%、87.8%。它们与绝对浓度法的符合率分别为利福平:0.746;异烟肼:0.750。结论 PhaB-NRA法简化了实验步骤、缩短了药敏检测时间,且无需昂贵的仪器,有望成为快速结核杆菌药物敏感性测定的初筛方法。

关键词: 结核分枝杆菌, 分枝杆菌噬菌体, 硝酸盐还原实验, 抗结核药物, 药物敏感性实验

Abstract: ABSTRACT:Objective To establish a rapid, inexpensive, and simple drug susceptibility test (DST ) for Mycobacterium tuberculosis (M.tb) and evaluate its feasibility. MethodWe used nitrate reductase combined with mycobacteriophage assay( PhaB-NRA) to test 49 clinical M.tb isolates of, and the results were compared with those of PhaB-NRA and traditional absolute concentration method. Results The sensitivity, specificity, and accuracy of PhaB-NRA for rifampicin were 89.1%, 91.67%, and 89.8%; on the contrary, those of isonicotinyl hydrazide were 86.21%, 90.0%, and 87.8%, respectively. The coincidence between PhaB-NRA and traditional assay were 0.746 for rifampicin and 0.750 for isonicotinyl hydrazide. Conclusions PhaB-NRA is an inexpensive, rapid, and simple DST method. It is a promising rapid screening technique for DST of M.tb.

Key words: mycobacterium tuberculosis, mycobacteriopdace, nitrate reductase, antituberculosis druc, druc susceptibility test