中国医学科学院学报

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中国医学科学院学报

中国医学科学院学报 ›› 2009, Vol. 31 ›› Issue (4): 423-426.doi: 10.3881/j.issn.1000-503X.2009.04.008

• 论著 • 上一篇    下一篇

结核分枝杆菌休眠菌的复苏培养与初始菌量的关系

刘忠泉;邢爱英;古淑香;贾红彦;张宗德   

  1. 北京市结核病胸部肿瘤研究所结核病分子生物学研究室,北京101149

  • 收稿日期:2009-02-12 修回日期:1900-01-01 出版日期:2009-08-30 发布日期:2009-08-30
  • 通讯作者: 张宗德

Relationship between the Resuscitation Promoting Role of Resuscitation Promoting Factor and the Initial Bacteria Amount of Dormant Mycobacterium Tuberculosis

LIU Zhong-quan; XING Ai-ying;GU Shu-xiang;JIA Hong-yan;ZHANG Zong-de   

  • Received:2009-02-12 Revised:1900-01-01 Online:2009-08-30 Published:2009-08-30
  • Contact: ZHANG Zong-de

摘要:

摘要:目的 探讨复苏因子对结核分枝杆菌休眠菌复苏作用与初始菌量的关系。方法 取培养100d的陈旧结核分枝杆菌(休眠菌),用7H9稀释成麦氏比浊1mg/ml,之后倍比稀释成0.5、0.25、0.125、0.0625、0.03125mg/ml。取12支无菌培养管,加入5ml 7H9培养基并分为2组,每组依次加入上述各浓度结核分枝杆菌,第1组加入复苏因子蛋白质,第2组作为对照加入等体积7H9,37℃培养,分别在15、25、30、35d检测光密度值,并取1μl培养液涂7H11平板培养,进行菌落计数。重复上述实验。结果 0、15、25、30、35d分光光度计检测光密度(OD)值结果显示:各浓度组间细菌增殖呈直线正相关性(P<0.05,P<0.01),即复苏因子对不同稀释浓度的复苏作用相同。7H11培养结果和OD值检测结果显示:各组两种方法检测结果呈直线相关性(P<0.05, P<0.01),即两种方法检测结果有一致性。结论 复苏因子对结核分枝杆菌休眠菌的复苏作用与初始菌量无关。

关键词: 结核分枝杆菌, 复苏因子, 培养

Abstract: ABSTRACT:Objective To investigate the relationship between the resuscitation promoting role of resuscitation promoting factor and the initial bacteria amount of dormant Mycobacterium tuberculosis. Methods Mycobacterium tuberculosis (dormant bacteria) was cultured for 100 days, then diluted into 1 mg/ml concentration with 7H9, and further diluted into 0.5, 0.25, 0.125, 0.0625, and 0.03125 mg/ml. Twelve new tubes added with 5 ml 7H9 and divided into two groups: the first group was added with the resuscitation-promoting factor protein, and the second group as control was added with 7H9. In each group the above diluted solutions were added. The tubes were located at 37℃ for culture. Optical density (OD) was detected on day 15, 25, 30, and 35. From each tube 1 μl culture solution was plated on 7H11 medium for colony counting. Results OD detection showed that bacteria proliferation in each group had positive linear correlation (P<0.05, P<0.01), indicating that the resuscitation-promoting factor played a similiar role in solutions with different dilution concentrations. 7H11 results and the OD results show that these two detection methods in each group had linear correlation (P<0.05, P<0.01), indicating that these two methods showed consistent test results. Conclusion The resuscitation-promoting factor has no effect on the resuscitation of dormant Mycobacterium tuberculosis and its initial bacteria amount.

Key words: mycobacterium tuberculosis, resuscitation promotinc factor, culture