中国医学科学院学报

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中国医学科学院学报

中国医学科学院学报 ›› 2009, Vol. 31 ›› Issue (4): 449-452.doi: 10.3881/j.issn.1000-503X.2009.04.013

• 论著 • 上一篇    下一篇

评价γ干扰素释放分析T-SPOT.TB在肺外结核病诊断中的敏感性

霍霏霏;张丽帆;刘晓清   

  1. 中国医学科学院北京协和医学院北京协和医院感染科,北京100730
  • 收稿日期:2009-05-19 修回日期:1900-01-01 出版日期:2009-08-30 发布日期:2009-08-30
  • 通讯作者: 刘晓清

Sensitivity of Interferon-gamma Release Assay T-SPOT.TB in Diagnosing Extrapulmonary Tuberculosis

HUO Fei-fei;ZHANG Li-fan;LIU Xiao-qing   

  1. Department of Infectious Diseases, PUMC Hospital, CAMS and PUMC, Beijing 100730, China
  • Received:2009-05-19 Revised:1900-01-01 Online:2009-08-30 Published:2009-08-30
  • Contact: LIU Xiao-qing

摘要: 摘要:目的 评价γ干扰素释放分析T-SPOT.TB检测在肺外结核病诊断中的敏感性。方法 对北京协和医院根据细菌学或/和组织学诊断的肺外结核患者31例,应用酶联免疫斑点技术检测6kD早期分泌靶向抗原和10kD培养滤过蛋白肽段库刺激后外周血中释放γ-干扰素的特异性T 细胞。结果 31例肺外结核患者中29例T-SPOT.TB检测阳性,敏感性为93.5%(95% CI 84.8%~100%),6kD早期分泌靶向抗原肽段库刺激后斑点形成细胞数中位数为196/106 PBMCs(4分位间距72~532/106 PBMCs),10kD培养滤过蛋白肽段库刺激后斑点形成细胞数中位数为276/106PBMCs(4分位间距72~568/106 PBMCs),对两个肽段库总的斑点形成细胞中位数为612/106PBMCs(4分位间距192~1152/106 PBMCs)。结论 T-SPOT.TB检测对肺外结核诊断具有较高的敏感性。

关键词: 肺外结核, 敏感性, T-SPOT.TB, 6kD早期分泌靶向抗原肽段库, 10kD培养滤过蛋白肽段库, γ干扰素释放分析

Abstract: ABSTRACT:Objective To evaluate the sensitivity of an interferon-gamma release assay T-SPOT.TB in the diagnosis of bacteriologically or histologically confirmed extrapulmonary tuberculosis. MethodTotally 31 patients with bacteriologically or histologically confirmed extrapulmonary tuberculosis in Peking Union Medical College Hospital received T-SPOT.TB assay to detect early secreting antigen target 6 or culture filtrate protein 10 peptides-specific T cells in the peripheral blood mononuclear cells(PBMCs). Results T-SPOT.TB assay showed positive results in 29 patients with extrapulmonary tuberculosis and the sensitivity was 93.5%(95% CI 84.8% - 100%). The median of spot forming cells(SFCs) in response to early secreting antigen target 6 peptides was 196/106PBMCs (interquartile range, 72-532/106 PBMCs), the median of SFCs in response to culture filtrate protein 10 peptides was 276 SFCs/106 PBMCs (interquartile range, 72-568/106 PBMCs), and the median of the incorporate SFCs was 612/106PBMCs (interquartile range, 192-1152/106 PBMCs). Conclusion T-SPOT.TB is highly sensitive in diagnosing extrapulmonary tuberculosis.

Key words: extrapulmonary tuberculosis, sensitivity, t-spot.tb, early secretinc anticen tarcet 6 peptides, culture filtrate protein 10 peptides, interferon-camma release assay