中国医学科学院学报

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中国医学科学院学报

中国医学科学院学报 ›› 2009, Vol. 31 ›› Issue (4): 506-510.doi: 10.3881/j.issn.1000-503X.2009.04.025

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γ干扰素释放分析T-SPOT.TB诊断结核感染临床应用进展

张丽帆;刘晓清   

  1. 中国医学科学院北京协和医学院北京协和医院感染科,北京 100730
  • 收稿日期:2009-05-19 修回日期:1900-01-01 出版日期:2009-08-30 发布日期:2009-08-30
  • 通讯作者: 刘晓清

Advances in Clinical Applications of T-SPOT.TB for Interferon-gamma Release Assay in the Immunodiagnosis of Tuberculosis

ZHANG Li-fan;LIU Xiao-qing   

  1. Department of Infectious Diseases, PUMC Hospital, CAMS and PUMC, Beijing 100730, China
  • Received:2009-05-19 Revised:1900-01-01 Online:2009-08-30 Published:2009-08-30
  • Contact: LIU Xiao-qing

摘要: 摘要:T-SPOT.TB是一种γ干扰素释放分析,用酶联免疫斑点技术检测对6kD早期分泌靶向抗原和10kD培养滤过蛋白肽段库反应的T细胞以诊断结核感染。其具有高度的敏感性和特异性,不受机体免疫力及卡介苗接种的影响。在欧洲及美国,T-SPOT.TB已被批准应用于临床,其越来越多的研究用于鉴别活动性结核与潜伏性结核感染,预测结核发病风险等。本文主要探讨T-SPOT.TB的临床应用进展。

关键词: 结核免疫, 结核诊断, 活动性结核, 潜伏性结核, T-SPOT.TB, γ干扰素释放分析

Abstract: ABSTRACT:T-SPOT.TB is an interferon-gamma release assay to detect T-cell response to early secreting antigen target 6 and culture filtrate protein 10 peptides by enzyme-linked immunospot assay for tuberculosis diagnosis. It is highly sensitive and specific, and will not be affected by the subject’s immune status and Bacillus Calmette-Guerin vaccination. This assay has been licensed for in-vitro diagnosis in Europe and the United States. Its potential roles in distinguishing active tuberculosis from latent tuberculosis infection and predicting active tuberculosis among individuals with latent tuberculosis have been increasingly studies. This article reviews the advances in the clinical application of T-SPOT.TB.

Key words: tuberculosis immunolocy, tuberculosis diacnosis, active tuberculosis, latent tuberculosis, t-spot.tb, interferon-camma release assay