中国医学科学院学报

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中国医学科学院学报

中国医学科学院学报 ›› 2011, Vol. 33 ›› Issue (2): 185-188.doi: 10.3881/j.issn.1000-503X.2011.02.017

• 论著 • 上一篇    下一篇

系统性红斑狼疮患者外周血单个核细胞中微小RNA -146a的表达

王海燕1,李 扬1,陈梅红2,张 烜1   

  1. 1中国医学科学院 北京协和医学院 北京协和医院风湿免疫科,北京 100730 2中国医学科学院 北京协和医学院 基础医学研究所生物化学系, 北京 100005
  • 收稿日期:2010-05-04 出版日期:2011-04-10 发布日期:2011-04-10
  • 通讯作者: 张烜 电话:010-88068177,陈梅红 电话:010-67884240 E-mail:zxpumch2003@yahoo.com.cn
  • 基金资助:

    教育部新世纪优秀人才计划(NCET-04-0191),国家自然科学基金(30400410),北京自然科学基金(7052052)和国家重点基础研究发展计划项目子课题(2007CB512405)

Expression of MicroRNA-146a in Peripheral Blood Mononuclear Cells in Patients with Systemic Lupus Erythematosus

WANG Hai-yan1, LI Yang1, CHEN Mei-hong2 ,ZHANG Xuan1   

  1. 1Department of Rheumatology, PUMC Hospital, CAMS and PUMC, Beijing 100730, China 2Department of Biochemistry,Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 100005, China
  • Received:2010-05-04 Online:2011-04-10 Published:2011-04-10
  • Contact: ZHANG Xuan Tel:010-88068177, CHEN Mei-hong Tel: 010-67884240 E-mail:zxpumch2003@yahoo.com.cn
  • Supported by:

    the Ministry of Education's New Century Excellent Talents Scheme (NCET-04-0191), the National Natural Sciences Foundation of China(30400410), the Natural Sciences Foundation of Beijing(7052052), and the National Basic Research Program Sub-topics of China(2007CB512405)

摘要: 目的 采用生物信息学和分子生物学技术相结合,寻找在系统性红斑狼疮(SLE)中特异性表达的微小RNA,为进一步研究微小RNA在SLE发病机制中的作用打下基础。方法 查找与SLE发病相关的基因,采用微小RNA靶基因预测数据库预测SLE相关基因上的可能微小RNA靶位点,选择其相对应的微小RNA-146a,即hsa-miR-146a,采用实时荧光定量PCR方法检测SLE患者与正常人外周血单个核细胞(PBMCs)中微小RNA的表达差异。 结果 SLE患者外周血PBMCs中hsa-miR-146a的循环阈值的差值高于正常对照组(4.52±1.18 比2.76±1.38,P=0.02),hsa-miR-146a的表达量显著低于正常对照组。结论 SLE患者PBMCs中hsa-miR-146a的表达水平降低,表明hsa-miR-146a在SLE发病机制中可能发挥一定的作用。

关键词: 微小RNA, 系统性红斑狼疮, 外周血单个核细胞

Abstract: Objective To explore the expression pattern of microRNAs in the peripheral blood mononuclear cells (PBMCs) of patients with systemic lupus erythematosus (SLE), with an attempt to identify the role of microRNA in the pathogenesis of SLE. Methods SLE-related genes were searched from the published literatures. Using the microRNA target gene prediction databases, we predicted the putative microRNA targets in these SLE-related genes. For some of the corresponding microRNAs (hsa-miR-146a), quantitative real-time polymerase chain reaction was performed to determine the expression levels of these microRNAs in PBMCs of SLE patients (SLE group) and healthy controls (control group)Result The discrepancy of cycle threshold of hsa-miR-146a in PBMCs was significantly higher in SLE group (4.52±1.18) than in control group (2.76±1.38) (P=0.02), and the expression level of hsa-miR-146a was significantly lower in SLE group Conclusion The expression of hsa-miR-146a decreases in SLE patients, indicating that hsa-miR-146a may play a role in the pathogenesis of SLE.

Key words: microRNA, systemic lupus erythematosus, peripheral blood mononuclear cells

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