中国医学科学院学报

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中国医学科学院学报

中国医学科学院学报 ›› 2011, Vol. 33 ›› Issue (2): 210-213.doi: 10.3881/j.issn.1000-503X.2011.02.022

• 论著 • 上一篇    下一篇

三氯醋酸-丙酮沉淀法用于骨组织蛋白的提取

崔永锋1,杨 贞2,朱宝华1,肖鲁伟2,童培建2   

  1. 1杭州市萧山区第一人民医院骨科,杭州 311200 2浙江中医药大学 骨伤科研究所,杭州 310053
  • 出版日期:2011-04-10 发布日期:2011-04-10
  • 通讯作者: 崔永锋 电话:0571-83807651 E-mail:guke120@hotmail.com
  • 基金资助:

    中国博士后科学基金(20080431334)

Application of Trichloroacetic Acid-Acetone Precipitation Method for  Protein Extraction in Bone Tissue

CUI Yong-feng1, YANG Zhen2, ZHU Bao-hua1, XIAO Lu-wei2, TONG Pei-jian2   

  1. 1Department of Orthopedics, the First People's Hospital of Xiaoshan District, Hangzhou 311200, China 2Institute of Orthopedics, Zhejiang University of Traditional Chinese Medicine, Hangzhou 310053, China
  • Online:2011-04-10 Published:2011-04-10
  • Contact: CUI Yong-feng Tel:0571-83807651 E-mail:guke120@hotmail.com
  • Supported by:

    the China Postdoctoral Science Foundation(20080431334)

摘要: 目的 探讨三氯醋酸-丙酮沉淀法提取骨组织蛋白的可行性和效率。方法 采用盐酸脱钙法和三氯醋酸-丙酮沉淀法,分别用于骨组织蛋白的提取,并对两种方法的提取效率进行对比。结果 三氯醋酸-丙酮沉淀法可获得较高的骨蛋白提取率,与盐酸脱钙法相比,分子条带分布范围相近,且不受骨髓造血组织影响。结论 三氯醋酸-丙酮沉淀法可以用于骨组织蛋白质组的研究。

关键词: 三氯醋酸-丙酮沉淀法, 骨组织, 蛋白提取

Abstract: Objective To explore the feasibility and efficiency of extracting protein from bone tissue using trichloroacetic acid (TCA)-acetone precipitation method. Methods Hydrochloric acid (HCL) decalcification method and TCA-acetone precipitation method were separately used for bone protein extraction. The efficiencies of these two methods were compared. Results TCA-acetone precipitation method had significantly higher extraction efficiency. Compared with HCL decalcification method, it had less pollution from bone marrow hematopoietic tissue. Protein band distribution was similar between these two methods. Conclusion TCA-acetone precipitation method is useful for bone proteomics research.

Key words: trichloroacetic acid-acetone precipitation method, bone tissue, protein extraction

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