中国医学科学院学报

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中国医学科学院学报

中国医学科学院学报 ›› 2011, Vol. 33 ›› Issue (4): 357-361.doi: 10.3881/j.issn.1000-503X.2011.04.002

• 论著 • 上一篇    下一篇

燃煤型氟中毒对大鼠睾丸组织氧化应激效应的影响

肖跃海1, 孙发1, 李崇斌1, 石家齐1, 谷江1, 谢春2, 官志忠3, 于燕妮4   

  1. 1贵阳医学院附属医院泌尿外科,贵阳 550004
    2贵阳医学院公共卫生学院,贵阳 550004
    3贵阳医学院分子生物学实验室,贵阳 550004
    4贵阳医学院附属医院病理科,贵阳 550004
  • 收稿日期:2011-04-20 修回日期:2011-09-05 出版日期:2011-08-01 发布日期:2011-08-01
  • 通讯作者: 孙发 E-mail:sfguizhou@163.com
  • 基金资助:

    国家自然科学基金(30760253)和科技部国际合作重大项目(2010DFB30530)

Effect of Endemic Fluoride Poisoning Caused by Coal Burning on the Oxidative Stress in Rat Testis

XIAO Yue-hai1, SUN Fa1, LI Chong-bin1, SHI Jia-qi1, GU Jiang1, XIE Chun2, GUAN Zhi-zhong3,YU Yan-ni4   

  1. 1Department of Urology, Affiliated Hospital of Guiyang Medical College, Guiyang 550004, China
    2School of Public Health, Guiyang Medical College, Guiyang 550004,China
    3Department of Molecular Biology, Guiyang Medical College, Guiyang 550004, China
    4Department of Pathology, Affiliated Hospital of Guiyang Medical College, Guiyang 550004, China
  • Received:2011-04-20 Revised:2011-09-05 Online:2011-08-01 Published:2011-08-01
  • Contact: SUN Fa E-mail:sfguizhou@163.com
  • Supported by:

    Supported by the National Natural Science Foundation of China (30760253) and International Cooperation Ministry of Science and key projects(2010DFB30530)

摘要: 目的 研究燃煤型氟中毒对大鼠睾丸组织氧化效应的影响,探讨燃煤型氟中毒的生殖毒害机制。方法 将40只雄性SD大鼠随机分为对照组、低氟组、中氟组、高氟组4组,每组10只。各染氟组喂饲含不同比例的燃煤型氟中毒病区煤烘玉米的饲料,构建燃煤型氟中毒动物模型。分别于120、180-d分批处死,各时间点每组处死大鼠数为5只。采用HE染色,光镜下观察睾丸组织病理学改变;制备睾丸组织匀浆,检测睾丸组织中丙二醛(MDA)含量及超氧化物歧化酶(SOD)、总一氧化氮合酶(T-NOS)和诱导型一氧化氮合酶(iNOS)活性;氟离子选择电极法检测大鼠尿氟含量及睾丸组织氟含量。结果 成功建立大鼠氟中毒模型。各染氟组大鼠睾丸组织氟含量均明显升高(P均<0.01),睾丸生精小管均发生不同程度的损害。120-d和180-d时,各染氟组大鼠睾丸组织T-NOS、iNOS活性和MDA含量均明显升高(P<0.05或P<0.01),且随染氟剂量增加而升高;120-d和180-d时,各染氟组大鼠睾丸SOD活性较对照组明显降低(P<0.05或P<0.01)。结论 燃煤型氟中毒可导致大鼠睾丸内氧化系统与抗氧化系统失衡,生殖细胞受损。氧化应激损伤在燃煤型氟中毒所致的雄性大鼠生殖毒性中可能发挥重要作用。

关键词: 燃煤型氟中毒, 睾丸, 氧化应激

Abstract: Objective To explore the effect of endemic fluoride poisoning caused by coal burning on the oxidative stress in rat testis. Methods Totally 40 male SD rats were equally randomized into four groups control group, low fluororsis group, middle fluororsis group, and high fluorosis group. Rats in all three fluororsis groups were fed with corn dried by burning coal obtained from endemic fluorosis areas with high fluoride, and thus the animal models of fluorosis were established. After 120 and 180 days, all the rats were sacrificed. Testis tissues were stained with hematoxylineosin and observed under light microscope. The malonaldehyde (MDA) content, superoxide dismutase (SOD) activity, total nitric oxide synthase (TNOS), and iducible nitric oxidase synthase (iNOS) were measured by biochemical methods in the testis tissues. The content of NaF in testis was measured by fluorine selective electrode. Results The rat fluororsis models were successfully established. The fluoride content in testis was significantly increased in all the fluororsis groups(P<0.01). Testicular structures were damaged in all of fluoride groups. The TNOS, iNOS activities, and MDA content of each fluoride group were significantly higher than that of the control group on day 120 and 180 (P<0.05 or 0.01 ). The TNOS, iNOS activities, and MDA content significantly increased in a dosedependent manner (P<0.05 or 0.01). The SOD activities significantly decreased in all the fluoride groups (P<0.05 or 0.01). Conclusions Endemic fluoride poisoning caused by coal burning can cause disorders in the oxidative system and antioxidative system in rat testis. The oxidative stress may play an important role in the fluoridesinduced reproductive toxicity in male rats.

Key words: endemic fluorosis poisoning caused by coal burning, testis, oxidative stress

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