中国医学科学院学报

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中国医学科学院学报

中国医学科学院学报 ›› 2017, Vol. 39 ›› Issue (2): 215-224.doi: 10.3881/j.issn.1000-503X.2017.02.009

• 论著 • 上一篇    下一篇

低频脉冲超声对失下牙槽神经后下颌骨病理改变修复的影响

杨博, 吴庆庆, 张亮, 郭彦君, 宫苹()   

  1. 四川大学 华西口腔医学院口腔疾病研究国家重点实验室 华西口腔医院种植科,成都 610041
  • 收稿日期:2016-12-25 出版日期:2017-04-20 发布日期:2017-04-20
  • 作者简介:

    通信作者:宫 苹 电话:028-85501427,电子邮件:gp602002@163.com

  • 基金资助:
    国家自然科学基金(81571008、81500895)

Effect of Low-intensity Pulsed Ultrasound on the Mandibular Remodeling Following Inferior Alveolar Nerve Transection

Bo YANG, Qingqing WU, Liang ZHANG, Yanjun GUO, Ping GONG()   

  1. State Key Laboratory of Oral Disease,West China School of Stomatology,Department of Oral Implantology,West China Hospital of Stomatology,Sichuan University,Chengdu 610041,China
  • Received:2016-12-25 Online:2017-04-20 Published:2017-04-20
  • Supported by:
    Supported by the National Nature Sciences Fundation of China (81571008,81500895)

摘要:

目的 观察低频脉冲超声对于神经损伤导致的骨质病变的修复效果,并探讨超声作用的最佳剂量。方法 采用外科手术法经颊侧入路截断大鼠下牙槽神经,对接缝合后每日行低频脉冲超声干预,术后4周行下颌骨取材,进行感觉功能的恢复测试,观察神经恢复情况,显微CT定量观察骨质改变,并通过HE、Masson以及免疫组织化学分析病理变化。结果 截断神经后超声干预可以促进神经损伤的恢复,同时提高骨小梁厚度与连续性,且占空比越高,效果越明显。结论 低频脉冲超声可促进神经损伤导致的骨质病变修复。

关键词: 低频脉冲超声, 下牙槽神经, 下颌骨

Abstract:

Objective To investigate the effects of low-intensity pulsed ultrasound (LIPUS) on the mandibular remodeling following inferior alveolar nerve transection (IANX) and to optimize the parameters of LIPUS in the treatment of nerve injury. Methods IANX was performed in male Sprague-Dawley rats. Four weeks after IANX,the effect of daily LIPUS (from day 1) on the transected inferior alveolar nerve was examined in terms of sensitivity to mechanical stimulation. Moreover,histopathologic changes of mandibles were analyzed by micro-CT,HE staining,Masson trichrome staining,and immunohistochemical staining. Results LIPUS promoted the recovery of inferior alveolar nerve injury after transection. HE staining displayed the improvement of trabecular thickness and continuity. LIPUS with higher duty ratios had more obvious effect on bone remodeling. Conclusion LIPUS promotes the mandibular remodeling following IANX.

Key words: low-intensity pulsed ultrasound, inferior alveolar nerve, mandibular

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