中国医学科学院学报

高级检索
中国医学科学院学报

中国医学科学院学报 ›› 2007, Vol. 29 ›› Issue (3): 312-317.

• 论著 • 上一篇    下一篇

生物发光显像技术对血管生成抑制因子vasostatin在肿瘤细胞PC3中活性监测

胡洁淼,邱飞婵,阴 彬,龚燕华,袁建刚,强伯勤,王世真,彭小忠   

  1. 中国医学科学院 北京协和医学院 基础医学研究所医学分子生物学国家重点实验室国家人类基因组北方基因中心,北京 100005
  • 收稿日期:2007-01-15 修回日期:1900-01-01 出版日期:2007-06-30 发布日期:2007-06-30
  • 通讯作者: 彭小忠

Bioluminescent Imaging Monitoring of a Anti-angiogenesis
Therapeutic Gene vasostatin in Tumor Cell PC3

HU Jie-miao, QIU Fei-chan, YIN Bin, GONG Yan-hua, YUAN Jian-gang, QIANG Bo-qin, WANG Shi-zhen, PENG Xiao-zhong   

  1. National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, CAMS and PUMC; Chinese National Human Genome Center at Beijing, Beijing 100005, China
  • Received:2007-01-15 Revised:1900-01-01 Online:2007-06-30 Published:2007-06-30
  • Contact: PENG Xiao-zhong

摘要: 摘要:目的 应用非侵入性活体显像技术研究血管生成抑制因子vasostatin。方法 采用融合表达方案,将治疗基因 vasostatin和报告基因fluc偶联构建融合表达载体,使表达的融合蛋白中两个蛋白互相不干扰,并有天然的特性。结果 将稳定表达FLuc阳性对照和V10FL融合蛋白的PC3细胞进行体外生物发光显像。用稳定表达Fluc的PC3细胞构建荷瘤模型,用生物发光显像能检测到肿瘤的发生。结论 可应用非侵入性活体显像技术进行体内和体外基因表达的检测与监控。

关键词: 生物发光显像, vasostatin, 血管生成

Abstract: ABSTRACT:Objective To generate a sensitive tool for noninvasive monitoring of a therapeutic gene vasostatin. Methods We fused the bioluminescent reporter gene firefly luciferase to the therapeutic transgene vasostatin and ensured that these two proteins would not interrupt each other and kept their own natural character. Results We therefore examined clones of PC3 cells stably expressing fusion gene and positive control fluc with bioluminescence. In vivo imaging of PC3-Fluc subcutaneous tumors showed that the mean tumor bioluminescence increased in animals over several weeks. Conclusion Noninvasive monitoring facilitates the detection of gene expression in vivo and in vitro.

Key words: bioluminescent imacinc, vasostatin, anciocenesis