中国医学科学院学报

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中国医学科学院学报

中国医学科学院学报 ›› 2007, Vol. 29 ›› Issue (3): 318-323.

• 论著 • 上一篇    下一篇

人睾丸组织特异表达新基因HSD-9的克隆及其编码蛋白质功能

王 勇,陈轶男,乔 原,缪时英,王琳芳   

  1. 中国医学科学院 北京协和医学院 基础医学研究所医学分子生物学国家重点实验室, 北京 100005
  • 收稿日期:2007-01-15 修回日期:1900-01-01 出版日期:2007-06-30 发布日期:2007-06-30
  • 通讯作者: 王琳芳

Characteristics of a Novel Human Testis-specific Gene, HSD-9, and Its Encoding Protein

WANG Yong,CHEN Yi-nan,QIAO Yuan,MIAO Shi-ying,WANG Lin-fang   

  1. National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 100005, China
  • Received:2007-01-15 Revised:1900-01-01 Online:2007-06-30 Published:2007-06-30
  • Contact: WANG Lin-fang

摘要: 摘要:目的 利用本室建立的人睾丸组织中不同细胞特异表达的ESTs文库克隆新基因HSD-9,初步研究HSD-9蛋白的特性及功能。方法 采用电子克隆手段获得新基因HSD-9,生物信息学手段分析基因及编码蛋白质的特点,Northern blot研究HSD-9基因的表达谱,原核表达方法得到HSD-9蛋白并制备特异性多克隆抗体,免疫荧光结合激光共聚焦显微镜技术研究HSD-9蛋白在生精细胞中的定位及在哺乳动物细胞中的表达特点。结果 HSD-9基因在人睾丸组织特异表达,其大鼠同源物可在大鼠各级生精细胞中表达;HSD-9- EGFP在CHO细胞和S4细胞中表达呈现沿细胞膜分布的小颗粒和偏心分布于胞内一侧的粗大颗粒; HSD-9-EGFP和网格蛋白clathrin可以部分共定位。结论 HSD-9是人睾丸组织特异表达新基因,其编码蛋白质在CHO细胞和S4细胞中表达特点非常类似于内吞小体蛋白的表达特点,推测其可能参与了细胞中内吞过程的调节。

关键词: HSD-9, 人睾丸组织特异表达基因, clathrin, 内吞

Abstract: ABSTRACT:Objective To study the characteristics of a novel human testis-specific gene, HSD-9, and its encoding protein. Methods HSD-9 was a novel gene from a human germ cells-specific ESTs library established in our laboratory. We used an electronic cloning method to obtain HSD-9 gene, and analyzed the characteristics of this novel gene and encoding product by bioinformatics methods, detected its expressing profile using a Northern blot assay, prepared specific rabbit polyclonal antibodies against HSD-9 protein, observed the localization of this protein in the germ cells and some somatic cells with confocal microscopy. Results HSD-9 was expressed in human testes, and its rat homolog was found in the varying germ cells. HSD-9 protein could partly be colocalized with clathrin. Conclusions HSD-9 is specific in human testes, and the expression pattern of its encoding product is similar to those of some endocytosis proteins. It is speculated that HSD-9 protein may function in the endocytosis.

Key words: dsd-9, duman testis-specific cene, clatdrin, endocytosis