中国医学科学院学报

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中国医学科学院学报

中国医学科学院学报 ›› 2007, Vol. 29 ›› Issue (3): 379-383.

• 论著 • 上一篇    下一篇

猴免疫缺陷病毒感染的猴自身抗体和自身免疫

卢耀增1,2,吴小闲1,2,符林春1,罗红梅1,陈 颂1,郭卫中1,邓文娣1,周映云1,赖春辉1   

  1. 1广州中医药大学 热带医学研究所,广州 510405
    2中国医学科学院 北京协和医学院 实验动物研究所,北京 100021
  • 收稿日期:2006-08-07 修回日期:1900-01-01 出版日期:2007-06-30 发布日期:2007-06-30
  • 通讯作者: 卢耀增

Autoantibodies and Autoimmunity in Simian Immunodeficiency Virus-infected Monkeys

LU Yao-zeng1,2, WU Xiao-xian1,2, FU Lin-chun1, LUO Hong-mei1, CHEN Song1,GUO Wei-zhong1, DENG Wen-di1, ZHOU Ying-yun1, LAI Chun-hui1   

  1. 1Institude of Tropical Medicine, Guangzhou University of Traditional Chinese Medicine, Guangzhou 510405, China
    2 Institude of Laboratory Animal Science, CAMS and PUMC, Beijing 100021, China
  • Received:2006-08-07 Revised:1900-01-01 Online:2007-06-30 Published:2007-06-30
  • Contact: LU Yao-zeng

摘要: 摘要:目的 研究猴免疫缺陷病毒(SIV)感染的猴艾滋病模型自身抗体和病理形态学的动态变化,探讨艾滋病与自身免疫的关系。方法 采用间接免疫荧光法,以小鼠心、肝、脾、肺、肾和淋巴结为靶抗原测定SIV感染猴各时间点的自身抗体;采用ECV304内皮细胞株和粒细胞抗原片分别测定抗内皮细胞和粒细胞的自身抗体。并对比检查相应的淋巴结、肾、脑等脏器的病理组织学改变。结果 SIV感染后多种自身抗体比感染前升高,如抗淋巴细胞抗体、抗血管内皮细胞抗体和抗粒细胞抗体等。病理组织学检查结果显示,在淋巴结有不同程度的组织损伤;在淋巴结、大脑皮层、消化道黏膜下层、心脏间质、肾和肝窦等脏器内有比较明显的小血管病变及其诱发的病理改变。结论 猴自身抗体水平的升高及相应组织、器官的损伤是猴艾滋病自身免疫的佐证。

关键词: 猴免疫缺陷病毒, 自身抗体, 自身免疫

Abstract: ABSTRACT:Objective To study the relationship between simian acquired immunodeficiency syndrome (SAIDS) and autoimmunity in simian immunodeficiency virus (SIV)-infected monkeys. Methods Indirect immunofluorescence assays were performed to detect plasma or serum autoantibodies in SIV-infected monkeys. The heart, liver, spleen, lung, kidney, and lymph node of BALB/c mice, a strain of endothelial cell ECV304, and granulocytes were used as target antigens. These results were compared with HE stained slides of SIV-infected monkeys. Results The levels of various autoantibodies, including anti-lymphocyte autoantibodies, anti-endothelial cell autoantibodies, and anti-granulocyte antibodies, increased after SIV infection in monkeys. Moreover, pathological examinations showed injuries in the lymphoid tissue and vascular pathological changes in cerebral cortex, submucosa of gastrointestinal tract, interstitial capillaries of myocardium, nephron of the kidney, and sinusoid cell of liver. Conclusion The increased autoantibodies and the pathological changes of tissues and organs confirm the existence of autoimmunity in SIV-infected monkeys.

Key words: simian immunodeficiency virus, autoantibody, autoimmunity