中国医学科学院学报

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中国医学科学院学报

中国医学科学院学报

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桂枝茯苓胶囊对放置宫内节育器模型大鼠血液流变学的调控及子宫平滑肌细胞表型蛋白表达的影响

卢颖1, 朱颖军2, 岳秀英1, 陈绍正1, 王雄3   

  1. 1天津医科大学宝坻临床医院妇科,天津 301800;
    2天津中心妇产科医院普妇科,天津 300100;
    3天津医科大学宝坻临床医院超声科,天津 301800
  • 收稿日期:2017-06-09 出版日期:2018-05-03 发布日期:2018-05-03
  • 通讯作者: 朱颖军 电话:022-58287129,电子邮件:zhuyj8072@sina.com

Effects of Gui Zhi Fu Ling Jiang Nang on Hemorheology, Expression of Phenotypic Proteins in Uterine Smooth Muscle Cells in Rats with An Intrauterine Device

LU Ying1, ZHU Yingjun2, YUE Xiuying1, CHEN Shaozheng1, WANG Xiong3   

  1. 1Department of Gynecology,Baodi Clinical College of Tianjin Medical University,Tianjin 301800, China;
    2Department of General Gynecology,Tianjin Central Hospital of Gynecology Obstetrics,Tianjin 300100, China;
    3Department of Ultrasonography, Baodi Clinical College of Tianjin Medical University Tianjin 301800, China
  • Received:2017-06-09 Online:2018-05-03 Published:2018-05-03
  • Contact: ZHU Yingjun Tel: 022-58287129,E-mail:zhuyj8072@sina.com

摘要: 目的 研究桂枝茯苓胶囊对放置宫内节育器(IUD)模型大鼠子宫平滑肌细胞(VSMC)表型标志物α-平滑肌肌动蛋白(α-SMA)和增殖细胞核抗原(PCNA)表型蛋白表达的影响及对大鼠外周血血栓素B2(TXB2)和6-酮-前列腺素F1α(6-keto-PGF1α)浓度的影响。方法 以Wistar雌性大鼠为实验对象,随机分为正常组(n=16)、模型对照组(n=18)、桂枝茯苓胶囊组(n=18)和氨基己酸片组(n=17)4组,分别给予正常饲养不处理,IUD建模后灌服0.9%生理盐水、氨基己酸片溶液和桂枝茯苓流浸膏处理,采用SP免疫组织化学法检测各组大鼠子宫内膜VSMC表型标志物α-SMA和PCNA的表达,ELISA法检测各组大鼠外周血血栓素B2(TXB2)和6-酮-前列腺素F1α(6-keto-PGF1α)的浓度。结果 正常组、模型对照组、桂枝茯苓胶囊组和氨基己酸片组大鼠子宫内膜α-SMA的阳性表达率分别为(50.89 ±9.41)%、(26.93 ±6.80)%、(48.92±6.80)%和(34.63±7.26)%,其中,正常组(t=14.43,P=0.00)和桂枝茯苓胶囊组(t=11.37,P=0.00)明显高于模型对照组,氨基己酸片组明显低于正常组(t=9.96,P=0.00)和桂枝茯苓胶囊组(t=8.23,P=0.00)。正常组、模型对照组、桂枝茯苓胶囊组和氨基己酸片组大鼠子宫内膜中PCNA的阳性表达率分别为(25.66±7.24)%、(61.26±9.98)%、(28.36±9.17)%和(50.23±8.71)%,其中,正常组(t=20.86,P=0.00)和桂枝茯苓胶囊组(t=19.12,P=0.00)明显低于模型对照组,氨基己酸片组明显高于正常组(t=17.82,P=0.00)和桂枝茯苓胶囊组(t=16.05,P=0.00)。正常组、模型对照组、桂枝茯苓胶囊组和氨基己酸片组大鼠的血清TXB2浓度分别为(445.86±24.43)、(508.78±12.42)、(448.11±9.63)和(498.11±13.63)ng/L,其中,模型对照组明显高于正常组(t=16.55,P=0.00)和氨基己酸片组(t=﹣4.12,P=0.00),桂枝茯苓胶囊组明显低于模型对照组(t=﹣15.23,P=0.00)和氨基己酸片组(t=﹣12.08,P=0.00)。正常组、模型对照组、桂枝茯苓胶囊组和氨基己酸片组大鼠的血清6-keto-PGF1α浓度分别为(23.17±1.93)、(18.09±0.93)、(22.70±1.61)和(20.70±1.41)ng/L,其中,模型对照组明显低于正常组(t=﹣13.98,P=0.00)和氨基己酸片组(t=5.26,P=0.00),桂枝茯苓胶囊组明显高于模型对照组(t=11.43,P=0.00)和氨基己酸片组(t=8.76,P=0.00)。结论 桂枝茯苓胶囊可调节IUD 模型大鼠子宫内膜α-SMA和PCNA 表达及血清TXB2和6-keto-PGF1α浓度。

关键词: 放置宫内节育器模型大鼠, 桂枝茯苓胶囊, α-平滑肌肌动蛋白;, 增殖细胞核抗原, 血栓素B2, 6-酮-前列腺素α

Abstract: Objective To observe the effect of Gui Zhi Fu Ling Jiang Nang (GZFLJN) on the expressions of alpha smooth muscle actin (α-SMA) and proliferating cell nuclear antigen (PCNA) in uterine vascular smooth muscle cells (VSMC) of rat models with an intrauterine device (IUD) and to determine the thromboxane B2 (TXB2) and 6-keto-prostaglandin F1α (6-keto-PGF1α) levels in peripheral blood. Methods Female Wistar rats were randomly divided into four groups: normal group (n=16, with normal breed without treatment), model group (n=18, drenching 0.9% normal saline after modeling of IUD), GZFLJN group (n=18), and aminocaproic acid tablets group (n=17). Immunohistochemical SP (steptavidin -perosidase) method was used to detect the expressions of a-SMA and PCNA in uterine VAMC. Enzyme-linked immunosorbent assay (ELISA) was served to detect the levels of TXB2 and 6-keto-PGF1α in peripheral blood. Results The positive rate of a-SMA were (50.89±9.41)%, (26.93±6.80)%, (48.92±6.80)%, and (34.63±7.26)%, respectively, in normal group, model group, GZFLJN group, and aminocaproic acid tablets group; obviously, it was significantly higher in normal group (t=14.43,P=0.00) and GZFLJN group (t=11.37,P=0.00) than that in model group and it was significantly lower in aminocaproic acid tablets group than in normal group (t=9.96,P=0.00) and GZFLJN group (t=8.23,P=0.00). The positive rate of PCNA were (25.66±7.24)%, (61.26±9.98)%, (28.36±9.17)%, and (50.23±8.71)%, respectively, in these four groups; obviously, it was significantly lower in the normal group (t=20.86,P=0.00) and GZFLJN group (t=19.12,P=0.00) than in model group and it was significantly higher in aminocaproic acid tablets group than in normal group (t=17.82,P=0.00) and GZFLJN group (t=16.05,P=0.00). Serum TXB2 level in these four groups were (445.86±24.43)ng/L, (508.78±12.42)ng/L, (448.11±9.63)ng/L, and (498.11±13.63)ng/L; obviously, it was significantly higher in model group than in normal group (t=16.55,P=0.00) and aminocaproic acid tablets group (t=﹣4.12,P=0.00) and it was significantly lower in GZFLJN group than in model group (t=-15.23,P=0.00) and aminocaproic acid tablets group (t=-12.08, P=0.00). Serum 6-keto-PGF1α level in these four groups were (23.17±1.93)ng/L, (18.09±0.93)ng/L, (22.70±1.61)ng/L, and (20.70±1.41)ng/L, respectively; obviously, it was significantly lower in model group than in normal group (t=﹣13.98,P=0.00) and aminocaproic acid tablets group (t=5.26, P=0.00) and it was significantly higher in GZFLJN group than in model group (t=11.43,P=0.00) and aminocaproic acid tablets group (t=8.76,P=0.00). Conclusion GZFLJN can regulate the expressions of a-SMA and PCNA of VSMC in the endometrium of IUD rats and the concentrations of TXB2 and 6-keto-PGF1α in the serum.

Key words: intrauterine device model rats, Guizhifuling capsule, α-smooth muscle actin, proliferating cell nuclear antigen, thromboxane B2, 6-keto-prostaglandin F1α

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