Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica ›› 2018, Vol. 40 ›› Issue (6): 769-777.doi: 10.3881/j.issn.1000-503X.10493

• Original Articles • Previous Articles     Next Articles

Validation and Evaluation of Diagnostic Efficiency of Genes Associated with Colorectal Cancer with Hyperglycemia

CUI Ge1,FENG Wenming2,ZHANG Ting3(),ZHU Guoliang1,SHI Qilin1,ZHANG Xiaolan1,XIA Hui1   

  1. 1Department of Pathology,Huzhou,Zhejiang 313000,China
    2Department of Surgery,First Affiliated Hospital of Huzhou University,Huzhou,Zhejiang 313000,China
    3Department of Pathology,Medical college,Huzhou University,Huzhou,Zhejiang 313000,China
  • Received:2018-04-23 Online:2018-12-20 Published:2019-01-03
  • Supported by:
    Supported by the Natural Sciences Foundation of Zhejiang Province(LQ16C080001);Public Welfare Technical Applied Research Project of Zhejiang Province Laboratory Animal Research(2017C37177);Medical Scientific Research Project of Zhejiang Province(2018KY775);Science and Technology Project of Huzhou City(2015GZ14)

Abstract:

Objective To verify the expressions of genes associated with colorectal cancer with hyperglycemia and evaluate their diagnostic values.
Methods Tumor tissues,distal normal intestinal mucosa,and peripheral blood samples were harvested from 109 colorectal cancer patients and peripheral blood samples from 30 diabetes patients and 30 healthy volunteers. The mRNA expressions of glucose regulated protein 78 (GRP78),NADPH oxidase-1 (NOX1),carcinoembryonic antigen-related cell adhesion molecule 5 (CEACAM5),heat shock protein 60 (HSP60),and histone deacetylase 1(HDAC1) were detected by real-time quantitative polymerase chain reaction. The correlation between the gene expressions and clinicopathological parameters in colorectal cancer patients were analyzed using Pearson’s correlation analysis. Diagnostic test accuracy evaluation was used to calculate the sensitivity,specificity,accuracy,predictability,Youden index,and likelihood ratio of serum gene expressions in colorectal cancer patients,and the receiver operating characteristic (ROC) curves were drawn. The area under the ROC curve was calculated to evaluate the diagnostic efficiency of the combined detection of multiple genes.
Results The mRNA levels of GRP78 (P=0.001),NOX1 (P=0.022),CEACAM5 (P=0.000),HSP60 (P=0.044),and HDAC1 (P=0.047) were positively correlated with the fasting blood glucose level. The mRNA expressions of NOX1 (P=0.000,P=0.008) and HDAC1 (P=0.000,P=0.037) in tissues and serum were significantly higher in colorectal cancer patients than in patients with normal blood glucose levels. The NOX1 mRNA expression was positively correlated with the diameter of colorectal cancer (P=0.013),and the HDAC1 mRNA expression was significantly correlated with the tumor site (P=0.049),depth of primary tumor invasion (P=0.025),and TNM stage (P=0.042). The areas under the ROC curves of NOX1,CEACAM5,and HDAC1 were 0.931,0.852,and 0.860 respectively (all P=0.000). The specificity,accuracy,and negative predictive value of NOX1,HDAC1 mRNA expression in colorectal cancer patients with hyperglycemia were all above 90%. The diagnostic sensitivity and specificity of the combined detection of NOX1,CEACAM5,and HDAC1 were 98.82% and 99.93%,respectively.
Conclusion Combined detection of genes associated with colorectal cancer accompanied by hyperglycemia can improve the diagnostic efficiency of early screening.

Key words: colorectal cancer with hyperglycemia, relative gene, glucose regulated protein 78, NADPH oxidase-1, carcinoembryonic antigen-related cell adhesion molecule 5, heat shock protein 60, histone deacetylase 1

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