硫化氢对大鼠肢体再灌注损伤后炎症因子和线粒体能量代谢障碍的影响

doi:10.3881/j.issn.1000-503X.10501

Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica ›› 2019, Vol. 41 ›› Issue (2): 234-241.doi: 10.3881/j.issn.1000-503X.10501

• Original Articles • Previous Articles Next Articles

Effects of Hydrogen Sulfide on Inflammatory Factors and Mitochondrial Energy Metabolic Disorders After Reperfusion Injury in Rats

YANG Yonghua¹(),WANG Wei²,HU Bin¹,YANG Hailong¹,WANG Xichun¹

1 Department of Orthopedics,Jiujiang First People’s Hospital,Jiujiang,Jiangxi 332000,China
2 Department of Orthopedics,the Second People’s Hospital Affiliated to Xiangya Medical College,Changsha 410008,China

Received:2018-04-26 Online:2019-04-28 Published:2019-05-07
Contact: Yonghua YANG E-mail:fengxiaokai1@126.com

Abstract

Abstract:

Objective To explore the effect of hydrogen sulfide on inflammatory factors and energy metabolism of mitochondria after limbs reperfusion injury in rats.Methods Sixty rats were divided into three groups:sham operation group,control group(ischemia-reperfusion injury + saline group),and experimental group(ischemia-reperfusion injury + H₂S group).Wistar rat models of limb ischemia-reperfusion injury were established.Skeletal muscle samples were collected to determine the levels of necrosis decomposition products [including myoglobin(MB),lipoprotein complex(LPC)and lipid peroxide(LPO)];blood samples were collected to determine the levels of interleukin(IL)-1,IL-6 and tumor necrosis factor-α(TNF-α);mitochondria were extracted for mitochondrial transmembrane potential measurement and ATP content detection.Statistical analysis was made on the test results.Results After ischemia reperfusion injury,the levels of MB,LPO,and LPC in skeletal muscle,liver,lung and renal tissues of the control group were significantly increased(MB:P_{skeletal muscle} =0.003,P_liver=0.001,P_lung =0.001,P_kidney =0.001;LPO:P_{skeletal muscle} =0.001,P_liver =0.001,P_lung =0.001,P_kidney =0.002;LPC:P_{skeletal muscle} =0.000,P_liver =0.002,P_lung =0.002,P_kidney =0.003),and hydrogen sulfide treatment during ischemia reperfusion significantly inhibited the production of MB,LPO,and LPC(MB:P_{skeletal muscle} =0.021,P_liver =0.036,P_lung =0.005;LPO:P_{skeletal muscle} =0.003,P_liver =0.008,P_lung =0.010,P_kidney =0.015;LPC:P_{skeletal muscle} =0.002,P_liver =0.026,P _lung =0.007,P _kidney =0.006).Ischemia/reperfusion of lower extremity in rats resulted in increased levels of IL-1,IL-6,and TNF-α in the serum of rats,and the levels of IL-1,IL-6,and TNF-increased over time,with statistically significant differences in IL-1,IL-6,and TNF-α among groups at 3 h(IL-1:P_{3 h} =0.019,P_{6 h} =0.011,P_{9 h} =0.009,$P_{12_{h}}$=0.008,and P_{15 h} =0.002;IL-6:P_{3 h} =0.026,P_{6 h} =0.009,P_{9 h} =0.002, $P_{12_{h}}$=0.002,P_{15 h} =0.003;TNF-α:P_{3 h} =0.002,P_{6 h} =0.002,P_{9 h} =0.005,$P_{12_{h}}$=0.002,P_{15 h} =0.003).The levels of IL-1,IL-6,and TNF-α in serum were significantly inhibited during ischemia reperfusion(IL-1:P_{3 h} =0.035,P_{6 h} =0.039,P_{9 h} =0.012,$P_{12_{h}}$=0.005,P_{15 h} =0.006;IL-6:P_{3 h} =0.042,P_{6 h} =0.025,P_{9 h} =0.023,$P_{12_{h}}$=0.006,P_{15 h}=0.005;TNF-α:P_{3 h} =0.005,P_{6 h} =0.003,P_{9 h} =0.022,$P_{12_{h}}$=0.005,P_{15 h} =0.005),and such inhibitory effects became even more obvious over time.After limb ischemia and reperfusion in the control group,the mitochondrial transmembrane potential of skeletal muscle cells significantly decreased compared with that of the sham group(t=6.698;P=0.001).After hydrogen sulfide treatment,the mitochondrial membrane potential energy of the experimental group was significantly higher than that of the control group(t=7.507,P = 0.000).The ATP level in the mitochondria of ischemia reperfusion rats in the control group was significantly lower than that in the sham group(t=7.526,P = 0.000).The content of mitochondrial ATP in the experimental group was significantly higher than that in the control group after hydrogen sulfide treatment(t=8.604,P = 0.000). Conclusions Hydrogen sulfide can alleviate the injury of skeletal muscle and distal organs after limb ischemia-reperfusion and reduce local inflammatory reaction.In addition,it is valuable in alleviating mitochondrial transmembrane potential and energy metabolism disorders during reperfusion injury.

Key words: ischemia-reperfusion injury, inflammatory factors, mitochondria, hydrogen sulfide

CLC Number:

R364.5

YANG Yonghua,WANG Wei,HU Bin,YANG Hailong,WANG Xichun. Effects of Hydrogen Sulfide on Inflammatory Factors and Mitochondrial Energy Metabolic Disorders After Reperfusion Injury in Rats[J].Acta Academiae Medicinae Sinica, 2019, 41(2): 234-241.

Figures/Tables 6

Table 1

Table 2

Table 3

Table 4

Table 5

Table 6

References 19

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分组Group	骨骼肌Skeletal muscle	肝脏Liver	肺脏Lung	肾脏Kidney
假手术组Sham operation group	0.63±0.12	0.47±0.07	0.52±0.06	0.77±0.10
对照组Control group	2.32±0.28	1.28±0.17	1.62±0.11	1.78±0.11
实验组Experience group	1.24±0.09	0.68±0.08	0.94±0.27	0.72±0.11
F	16.325	19.692	15.875	14.519
P	0.000	0.001	0.001	0.002
P1	0.003	0.001	0.001	0.001
P2	0.021	0.036	0.005	0.159

分组Group	骨骼肌Skeletal muscle	肝脏Liver	肺脏Lung	肾脏Kidney
假手术组Sham operation group	0.43±0.11	0.35±0.08	0.40±0.05	0.63±0.09
对照组Control group	2.02±0.25	1.15±0.19	1.42±0.18	1.58±0.12
实验组Experience group	0.84±0.09	0.71±0.07	0.62±0.07	0.62±0.08
F	16.522	13.985	14.050	16.160
P	0.001	0.002	0.001	0.002
P1	0.001	0.001	0.001	0.002
P2	0.003	0.008	0.010	0.015

分组Group	骨骼肌Skeletal muscle	肝脏Liver	肺脏Lung	肾脏Kidney
假手术组Sham operation group	0.73±0.09	0.75±0.08	0.65±0.07	0.83±0.08
对照组Control group	3.12±0.21	1.75±0.15	1.82±0.14	1.88±0.13
实验组Experience group	1.34±0.07	1.01±0.12	1.11±0.13	1.18±0.12
F	16.980	15.550	14.820	16.660
P	0.000	0.002	0.005	0.001
P1	0.000	0.002	0.002	0.003
P2	0.002	0.026	0.007	0.006

分组Group	0 h	3 h	6 h	9 h	12 h	15 h
假手术组Sham operation group	1.33±0.11	1.36±0.08	1.35±0.10	1.31±0.13	1.35±0.12	1.32±0.11
对照组Control group	1.31±0.11	1.58±0.09	1.63±0.07	1.77±0.10	1.83±0.11	1.92±0.10
实验组Experience group	1.34±0.12	1.47±0.06	1.40±0.09	1.48±0.08	1.41±0.09	1.38±0.07
F	2.362	7.650	9.996	10.526	10.231	12.659
P	0.305	0.012	0.003	0.002	0.002	0.001
P1	0.319	0.019	0.011	0.009	0.008	0.002
P2	0.296	0.035	0.039	0.012	0.005	0.006

分组Group	0 h	3 h	6 h	9 h	12 h	15 h
假手术组Sham operation group	1.33±0.11	1.33±0.10	1.35±0.06	1.31±0.09	1.35±0.12	1.32±0.11
对照组Control group	1.32±0.09	1.59±0.07	1.66±0.08	1.78±0.10	1.85±0.11	1.97±0.10
实验组Experience group	1.34±0.12	1.45±0.09	1.52±0.09	1.50±0.11	1.45±0.08	1.41±0.09
F	1.995	8.925	9.967	11.255	10.636	11.985
P	0.401	0.015	0.011	0.002	0.004	0.002
P1	0.415	0.026	0.009	0.002	0.002	0.003
P2	0.332	0.042	0.025	0.023	0.006	0.005

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Effects of Hydrogen Sulfide on Inflammatory Factors and Mitochondrial Energy Metabolic Disorders After Reperfusion Injury in Rats

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Figures/Tables 6

References 19

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分组Group	0 h	3 h	6 h	9 h	12 h	15 h
假手术组Sham operation group	0.91±0.09	0.94±0.08	0.82±0.09	0.83±0.08	0.81±0.08	0.83±0.09
对照组Control group	0.92±0.10	1.39±0.05	1.43±0.06	1.53±0.09	1.57±0.12	1.59±0.10
实验组Experience group	0.94±0.06	1.26±0.06	1.32±0.08	1.40±0.06	1.36±0.09	1.33±0.08
F	2.226	6.538	7.115	6.809	8.927	10.027
P	0.109	0.002	0.000	0.003	0.002	0.002
P1	0.425	0.002	0.002	0.005	0.002	0.003
P2	0.352	0.005	0.003	0.022	0.005	0.005

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