Acta Academiae Medicinae Sinica ›› 2019, Vol. 41 ›› Issue (2): 194-202.doi: 10.3881/j.issn.1000-503X.10674
• Original Articles • Previous Articles Next Articles
JI Chao1,MA Julei2,SHU Peng3()
Received:
2018-07-02
Online:
2019-04-28
Published:
2019-05-07
Contact:
Peng SHU
E-mail:961576995@qq.com
Supported by:
CLC Number:
JI Chao,MA Julei,SHU Peng. Effect of Denticleless E3 Ubiquitin Protein Ligase on Proliferation and Colone Formation of Multiple Myeloma Cells[J].Acta Academiae Medicinae Sinica, 2019, 41(2): 194-202.
Fig 1
DTL-shRNA infection and knock-down efficiencies in human multiple myeloma cell line RPMI8226 after RPMI8226 is infected by 10 folds of CON and DTL-shRNA lentivirus for 48 hours CON:control group;DTL-shRNA:denticleless E3 ubiquitin protein ligase-short hairpin RNA group;Mr:relative molecular mass A.detection of the infection efficiency by flow cytometry;B.detection of kock-down efficicency at protein level by Western blot"
Fig 2
DTL-shRNA inhibits RPMI8226 proliferation and colone formation and induces apoptosis and cell cycle arrest after RPMI8226 is infected by CON and DTL-shRNA lentivirus for 48 hours A.culture in semisolid medium for 10 days and detection of the change of colone formation using inverted phase contrast microscope(white arrow:classic colone or apoptosis cells);B.detection of the change of cell cycle by propidium iodide staining;C.detection of the change of cell apoptosis by annexin V/propidium iodide double staining"
Fig 3
Effect of DTL-shRNA on cell prolieration is related with NF-κB pathway alteration after RPMI8226 is infected by CON and DTL-shRNA lentivirus for 48 hours NF-κB:nuclear factor-κB;P-P65:phosphorylation P65;IκBα:inhibitory subunit-κBα;P-IκBα:phosphorylation IκBα;EMSA:electrophoretic mobility shift assay;OCT-1:organic cation transporter-1 A.detection of P65 and IκBα phosphorylation change in NF-κB pathway by Western blot;B.detection of the change of NF-κB transcriptional ability by EMSA"
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