Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica ›› 2019, Vol. 41 ›› Issue (1): 28-36.doi: 10.3881/j.issn.1000-503X.10840

• Original Articles • Previous Articles     Next Articles

Effect of Aging on Experimental Colitis in Mice and Its Associated Mechanism

LIU Ailing1,WANG Hongying2,Lü Hong1(),QIAN Jiaming1   

  1. 1 Department of Gastroenterology,PUMC Hospital,CAMS and PUMC,Beijing 100730,China
    2 Key Laboratory of Molecular Oncology,Cancer Hospital,CAMS and PUMC,Beijing 100021,China
  • Received:2018-09-11 Online:2019-02-28 Published:2019-03-06
  • Contact: Hong Lü E-mail:honglv2006@hotmail.com
  • Supported by:
    Supported by the Health Research & Special Projects Grant of China(201002020);the Chinese Academy of Medical Sciences Medicine and Health Technology Innovation Project(2016-I2M-3-001);the National Natural Sciences Foundation of China(81570505);2010 National Clinical Key Specialist Project and the National Science and Technology Ministry 973 Plan(2015CB943203)

Abstract:

Objective To explore whether aging increases severity of colitis in mice and its mechanism.Methods Young (6-8 weeks)and aged (56 weeks) C57Bl/6 mice were divided into the control and experimental group (n=5,each). Dextran sodium sulfate(DSS) was used to induce acute colitis mouse model in the experimental group.The mRNA expressions of tumor necrosis factor-α(TNF-α)and interleukin-6(IL-6)in colon were measured by RT-PCR. Tight junctions (TJs) of intestinal epithelial cells was examined by transmission electron microscopy (TEM). Protein expressions of E-cadherin and occludin were detected by Western blotting and immunohistochemistry in colon.Results Compared with the young DSS-induced mice,the aged DSS-induced mice had more weight loss(t=3.679,P=0.006),higher disease indexes (t=2.496,P=0.037),higher histologic scores(U=0.000,P=0.008) and higher colonic IL-6 level (U=4.000,P=0.191). The TJs of intestinal epithelial cells were discontinuous in old healthy rats,and the TJs were destroyed significantly in both young and aged DSS-induced mice. Compared with the young DSS-induced mice,the aged DSS-induced mice had decreased protein expressions of E-cadherin (t=0.184,P=0.863)and occludin (t=0.399,P=0.710).Conclusions Aging leads to more severe disease following DSS challenge. Age-related deterioration in the functions of the gastrointestinal barrier and integrity may be one of the possible mechanisms.

Key words: aging, inflammatory bowel disease, tight junction, intestinal barrier

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