钆赛酸二钠增强磁共振灌注与多期肝胆期成像定量评价大鼠肝纤维化

doi:10.3881/j.issn.1000-503X.11033

Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica ›› 2018, Vol. 40 ›› Issue (6): 809-816.doi: 10.3881/j.issn.1000-503X.11033

• Original Articles • Previous Articles Next Articles

Gadolinium Ethoxybenzyl Diethylene-triamine-pentaacetic-acid-enhanced Magnetic Resonance Imaging Perfusion and Multiple Hepatobiliary-phase Imaging for Quantitative Assessment of Liver Fibrosis in Rat Models

XU Jia¹,WANG Xuan¹(),YOU Yan²,XUE Huadan¹,WANG Qin¹,WANG Shitian¹,JIN Zhengyu¹

1Department of Radiology,Beijing 100730,China
2Department of Pathology,PUMC Hospital,CAMS and PUMC,Beijing 100730,China

Received:2018-11-22 Online:2018-12-20 Published:2019-01-03
Supported by:
Supported by the National Natural Sciences Foundation of China (Young Scientists Foundation)(81501446);the National Public Welfare Basic Scientific Research Program of Chinese Academy of Medical Sciences(2018PT32003);the National Public Welfare Basic Scientific Research Program of Chinese Academy of Medical Sciences(2017PT32004)

Abstract

Abstract:

Objective To investigate the value of gadolinium ethoxybenzyl diethylene-triamine-pentaacetic-acid (Gd-EOB-DTPA)-enhanced magnetic resonance imaging (MRI) in detecting different stages of liver fibrosis in rats.
Methods Rat models of liver fibrosis were induced by carbon tetrachloride intraperitoneal injection for 4 - 12 weeks (n=45). The control group was applied with 0.9% saline (n=15). The MRI protocol contained both dynamic contrast-enhanced sequence (60 continuous scans within 3 minutes,including three pre-contrast measurements) and multiple hepatobiliary-phase acquisitions (every 5 minutes after contrast injection,60 minutes in total). METAVIR score was used to grade liver fibrosis:normal (F0),mild fibrosis (F1 - F2),and advanced fibrosis (F3 - F4). Liver perfusion parameters [transfer constant (K ^trans),extravascular extracellular volume fraction (V_e),initial area under curve (iAUC),maximum relative enhancement (RE_max),and time of maximum RE (T_max)] as well as hepatobiliary-phase parameters [RE at different time point,the decrease of RE (RE_change=RE_{3 min} - RE_{60 min}),and elimination half-life of RE (T_RE1/2)] were measured and compared with ANOVA analysis and Spearman rank correlation.
Results Thirty-one rats completed MRI exams and were then divided into normal (n=10),mild fibrosis (n=10),and advanced fibrosis (n=11) groups. K ^trans,V_e and iAUC decreased as liver fibrosis progressed (r=-0.631,P=0.002;r=-0.503,P=0.017;r=-0.446,P=0.037). K ^trans and V_e showed significant differences among three groups(F=7.011,P=0.005;F=4.656,P=0.023). K ^trans and V_e were significantly lower in advanced fibrosis group than in normal group (P=0.001,P=0.009). There were statistical significant differences of T_max,T_RE1/2 and RE_change among groups(F=6.633,P=0.005;F=5.493,P=0.010;F=5.343,P=0.014). Compared to normal and mild fibrosis groups,advanced fibrosis group had significantly longer T_max and T_RE1/2 (P=0.005,P=0.004;P=0.008,P=0.008)and significantly lower RE_change(P=0.007,P=0.012).
Conclusion Perfusion and multi-hepatobiliary-phase parameters such as K ^trans,V_e,T^max, T_REI/2 and RE_change obtained from Gd-EOB-DTPA-enhanced MRI,may be valuable for detecting and staging liver fibrosis.

Key words: liver fibrosis, gadolinium ethoxybenzyl diethylene-triamine-pentaacetic-acid, magnetic resonance imaging, perfusion-weighted imaging, hepatobiliary phase

CLC Number:

R445.2

XU Jia,WANG Xuan,YOU Yan,XUE Huadan,WANG Qin,WANG Shitian,JIN Zhengyu. Gadolinium Ethoxybenzyl Diethylene-triamine-pentaacetic-acid-enhanced Magnetic Resonance Imaging Perfusion and Multiple Hepatobiliary-phase Imaging for Quantitative Assessment of Liver Fibrosis in Rat Models[J].Acta Academiae Medicinae Sinica, 2018, 40(6): 809-816.

Figures/Tables 5

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References 20

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肝纤维化Fibrosis	K^trans (min^-1)	K_ep(min^-1)	V_e(min^-1)	iAUC	T_max (s)	RE_max (%)
正常Normal(n=10)	0.727±0.162	1.760±0.316	0.449±0.093	15.356±3.704	150±33	97.32±13.86
轻度纤维化Mild fibrosis(n=10)	0.556±0.150	1.810±0.294	0.346±0.064^a	14.498±3.671	138±9	92.83±19.87
重度纤维化Advanced fibrosis(n=11)	0.448±0.155^b	1.510±0.355	0.332±0.081^c	11.098±5.239	189±58^de	82.57±19.51
F	7.011	1.916	4.656	2.253	6.633	1.903
P	0.005	0.193	0.023	0.100	0.005	0.169

肝纤维化Fibrosis	RE_{3 min} (%)	RE_{20 min}(%)	RE_{45 min} (%)	RE_{50 min} (%)	RE_{55 min} (%)	RE_{60 min} (%)	RE_下降(%)	T_RE1/2 (s)
正常 Normal(n=10)	83.39±13.86	32.22±18.11	14.57±7.94	7.07±10.61	6.08±5.99	4.01±4.67	91.49±11.39	722±156
轻度纤维化 Mild fibrosis(n=10)	80.41±18.35	30.25±18.86	10.44±6.64	7.22±5.46	5.51±6.04	4.78±4.06	89.76±20.20	718±110
重度纤维化 Advanced fibrosis(n=11)	77.75±28.98	45.94±23.76	24.51±14.48^e	24.06±15.48^f	23.78±15.35^ag	19.87±16.71^b	68.58±22.50^ch	1314±778^di
F	0.080	2.343	3.994	5.276	5.189	2.689	5.343	5.493
P	0.923	0.115	0.030	0.020	0.002	0.039	0.014	0.010

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Gadolinium Ethoxybenzyl Diethylene-triamine-pentaacetic-acid-enhanced Magnetic Resonance Imaging Perfusion and Multiple Hepatobiliary-phase Imaging for Quantitative Assessment of Liver Fibrosis in Rat Models

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