Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica ›› 2019, Vol. 41 ›› Issue (2): 149-155.doi: 10.3881/j.issn.1000-503X.11169

• Original Articles • Previous Articles     Next Articles

Neuromuscular Pharmacodynamics of Rocuronium in Diabetic Rats

LIU Xiaowen1,2,GONG Ruisong3,LIU Zhen4,ZUO Jun1,2,ZHAO Jing1,2()   

  1. 1 Graduate School,CAMS and PUMC,Beijing 100730,China
    2 Department of Anesthesiology,China-Japan Friendship Hospital,Beijing 100029,China
    3 Department of Anesthesiology,PUMC Hospital,CAMS and PUMC,Beijing 100730,China
    4 Department of Anesthesiology,Beijing Hospital,Beijing 100730,China
  • Received:2019-01-14 Online:2019-04-28 Published:2019-05-07
  • Contact: Jing ZHAO E-mail:zhaojing1009@aliyun.com
  • Supported by:
    Special Fund for Clinical Research of Chinese Medical Association(13081230508)

Abstract:

Objective To investigate diabetes-mediated changes in the neuromuscular pharmacodynamics of rocuronium in rats.Methods Diabetes mellitus was induced by a single injection of streptozotocin in rats.A total of 24 male SD rats were assigned to four groups using random number table:the normal control group,diabetic 2-week group,diabetic 4-week group,and diabetic 8-week group(6 rats per group).The sciatic nerve was stimulated in a rain-of-four(TOF)pattern,and the twitch tension changes in the tibialis anterior muscle were demonstrated by mechanomyography after intravenous injection of rocuronium in vivo.The time course characteristics of rocuronium,including onset time,and the recovery time from rocuronium injection to TOF ratio 75%(RT75%)and 90%(RT90%),were recorded,and half maximal inhibitory concentration(IC50)values of rocuronium were determined using a four-parameter dose response curve.Results Compared with the normal controls,the diabetic rats had significantly prolonged onset time of rocuronium,while the RT75% and RT90% were decreased at all rocuronium doses(P<0.001).The time course changes became increasingly significant as the duration of diabetes lengthened(P<0.001).The IC50 and 95% confidence interval values for rocuronium in the normal control group,diabetic 2-week group,diabetic 4-week group,and diabetic 8-week group were 0.37(0.35-0.38)mg/kg,0.44(0.43-0.46)mg/kg,0.59(0.57-0.61)mg/kg,and 0.64(0.61-0.66)mg/kg,respectively.IC50 values were significantly higher in the diabetic groups vs.normal control(P<0.001)and gradually increased as the duration of diabetes lengthened(P<0.001).Conclusion Diabetes is associated with the rat skeletal muscle hyposensitivity to rocuronium,which is featured by prolonged onset time of rocuronium,decreased RT 75% and RT 90%,and right shift of the cumulative dose-response curve of rocuronium.

Key words: diabetes mellitus, rocuronium, cumulative dose-response curve, half maximal inhibitory concentration, time course characteristics

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