Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica ›› 2008, Vol. 30 ›› Issue (5): 559-563.doi: 10.3881/j.issn.1000-503X.2008.05.011

• Original Articles • Previous Articles     Next Articles

Ferumoxide Labeled Flk1+ CD31- CD34-Human Bone Marrow Mesenchymal Stem Cells and Its in vivo Tracing in the Brains of Macaca Fascicularis

FENG Ming1,WANG Ren-zhi1,ZHU Hua2,ZHANG Nan3,WANG Chang-jun1,WEI Jun-ji1,LU Shan4, LI Qin2,YIN Xiao-ming3,HAN Qin4,MA Wen-bin1,QIN Chuang2,ZHAO Chun-hua4,AN Yi-hua5,KONG Yan-guo1   

  1. 1Department of Neurosurgery, PUMC Hospital, CAMS and PUMC, Beijing 100730, China
    2Department of Pathology, Institute of Labororatory Animal Sciences, CAMS and PUMC, Beijing 100021, China
    3Department of Neurosurgery, Coal General Hospital, Beijing 100018, China
    4Tissue Engineering center, Institute of Basic Medical Sciences ,CAMS and PUMC, Beijing 100730, China
    5Department of Neural Stem Cells, Institute of Neurosurgery of Beijing, Beijing 100050, China
  • Received:2007-10-12 Revised:1900-01-01 Online:2008-10-30 Published:2008-10-30
  • Contact: WANG Ren-zhi;ZHAO Chun-hua

Abstract: ABSTRACT:Objective To explore the method for labeling Flk1+ CD31- CD34- human bone marrow mesenchymal stem cells (hBMSCs) with ferumoxide-PLL and evaluate the feasibility of its tracing after transplantation into the brains of Macaca Fascicularis. Methods The hBMSCs were incubated with ferumoxide-PLL. Trypan blue staining, Prussian blue staining, and transmission electron microscope were performed to show intracellular iron, marking efficiency, and the vigor of the labeled cells. After the hBMSCs were transplanted into the brains of cynomolgus monkeys by stereotaxis, magnetic resonance imaging (MRI) was performed to trace the cells in vivo. Cell survival and differentiation were studied with immunohistochemistry, Prussian blue staining, and HE staining. Results The marking efficiency of the ferumoxide-PLL was 96%. Iron particles were found intracytoplamic of the hBMSCs by Prussian blue staining and transmission electron microscopy. The relaxation rates of labeled cells in MRI were 4.4 and 4.2 times higher than those of the unlabeled cells. Hypointensity area was found by MRI three weeks after transplantation. Many hBMSCs and new vessels were found in the transplantation zone by pathological and immunofluorescence methods. Conclusions Ferumoxide-PLL can effectively label hBMSCs and thus increase its contrast in MRI results. The cells can survive in the brains of cynomolgus monkeys. The labeled hBMSCs can be traced in vivo by MRI.

Key words: superparamacnetic iron oxide, mesencdymal stem cell, cell transplantation, macnetic resonance imacinc, macaca fascicularis