Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica ›› 2008, Vol. 30 ›› Issue (6): 659-662.doi: 10.3881/j.issn.1000-503X.2008.06.007

• Original Articles • Previous Articles     Next Articles

Effect of Vasopressin on Aquaporin 7 Expression in Rat Inner Ear

GU Feng-ming1;ZHANG Lian-shan2   

  1. 1Department of Otolaryngology, EYE and ENT Hospital, Fudan University, Shanghai 200031, China 2Department of Otolaryngology, PUMC Hospital, CAMS and PUMC, Beijing 100730, China
  • Received:2008-04-17 Revised:1900-01-01 Online:2008-12-30 Published:2008-12-30
  • Contact: GU Feng-ming

Abstract: ABSTRACT:Objective To study the effect of vasopressin on aquaporin 7 (AQP7) expression in rat inner ear and reveal the possible role of aquaporins in the formation of endolymphatic hydrops induced by vasopressin. Methods Wistar rats were intraperitoneally injected with 50 μg/kg arginine vasopressin once a day for one week. Differentially expressed genes of aquaporins induced by vasopressin injection in rat inner ear were filtered by cDNA microarray. The changes of mRNA expression level of AQP7 in inner ear of rats treated with vasopressin injection were measured by RT-PCR. Results Differentially expressed gene AQP7 of aquaporins induced by vasopressin injection was screened out in rat inner ear.The expression level of AQP7 mRNA in inner ear of rats treated with vasopressin injection was significantly lower. Conclusion Vasopressin may down-regulate the expression of AQP7 mRNA in the endolymphatic sac and induce a decreased absorption of endolymph, which decreases the water permeability in the potassium ions recycle pathway in the organ of Corti and disturbs the circulation of endolymph, resulting in endolymphatic hydrops.

Key words: vasopressin, inner ear, aquaporin 7, cdna microarray