Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica ›› 2009, Vol. 31 ›› Issue (3): 308-314.doi: 10.3881/j.issn.1000-503X.2009.03.015

• Original Articles • Previous Articles     Next Articles

Gene Expression Profile of Isoniazid Liver-injured Rat Using cDNA Micoarray LIAO Yan2, PENG Shuang-qing1, ZHANG Li-shi3

LIAO Yan2; PENG Shuang-qing1; ZHANG Li-shi3   

  1. 1Research and Evaluation Center for Toxicology, Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing 100071, China 2Department of Rehabilitation, School of Preclinical Medicine, Beijing University of Chinese Medicine, Beijing 100029, China 3Department of Nutrition and Food Hygiene, West China School of Public Health, Sichuan University, Chengdu 610041, China
  • Received:2008-07-24 Revised:1900-01-01 Online:2009-06-30 Published:2009-06-30
  • Contact: PENG Shuang-qing

Abstract: ABSTRACT:Objective To investigate the changes of gene expression profile of rat liver tissue by cDNA microarrays. Methods Twenty Wistar rats in control group(n=10) and isoniazid (INH) group (n=10)were orally administrated with normal saline and 400 mg/kg INH for 14 days, respectively. The differentially expressed genes significantly correlated with liver injury were screened and analyzed. The mechanisms of liver injury caused by INH were specifically analyzed at level of gene expression based on the biological functions of those differentially expressed genes. Results Thirty-seven differentially expressed genes were found in the rats administrated with INH. Among them, 25 genes were up-regulated, while the other 12 genes were down-regulated. These differentially expressed genes were functionally related to the changes of CYP450-related genes, fatty acid metabolism, and protein metabolism. Conclusion INH can cause the remarkable changes of the gene expression profiles in rat liver cells, which is important for further elucidating the mechanisms of liver injury caused by INH.

Key words: isoniazid, cene expression profile, depatotoxicity, rat