Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica ›› 2009, Vol. 31 ›› Issue (3): 322-325.doi: 10.3881/j.issn.1000-503X.2009.03.017

• Original Articles • Previous Articles     Next Articles

Method for Drainage of Lymph Fluid and Determining the Change of Active Materials in Lymph Fluid after Rat Ischemia-reperfusion Injury

CHEN Xue-feng1;HE Gui-zhen1;DONG Liang-guang1;SHU Hong1;WANG Xiu-rong1;GAO Feng-yan2   

  1. 1Department of Enteral and Parenteral Nutrition,PUMC Hospital,CAMS and PUMC,Beijing 100730,China 2 Division of NCD Control and Community Health, Chinese Center for Disease Control and Prevention, Beijing 100050,China
  • Received:2008-11-24 Revised:1900-01-01 Online:2009-06-30 Published:2009-06-30
  • Contact: HE Gui-zhen

Abstract: ABSTRACT:Objective To set up a method for the drainage of lymph fluid and explore the change of active materials in lymph fluid and serum after rat ischemia-reperfusion injury. Methods The method of the drainage of lymph fluid was well established. Sixteen healthy male rats of SPF grade were selected and randomly divided into 2 groups: intestinal ischemia-reperfusion + drainage group (I/R+drainage group) and drainage group. All the rats were subjected to superior mesenteric artery occlusion for 60 minutes, followed by 120 minutes of reperfusion. We compared the change of high mobility group box-1 (HMGB1) protein, endotoxin, tumor necrosis factor α (TNF-α), interleukin(IL)-1β, IL-6, and soluble vascular cell adhesion molecular-1 (sICAM-1) by draining lymph fluid and collecting serum in 2 groups. Results The drainage of lymph fluid was successfully performed. The HMGB1, endotoxin, and cytokines in serum and lymph fluid were significantly higher in ischemia-reperfusion group than in drainage group(P<0.05). Conclusions The method for drainage of lymph fluid is simple and feasible. Endotoxin, HMGB1, and some cytokines in serum and lymph fluid may mediate the ischemia-reperfusion injury.

Key words: intestine iscdemia reperfusion injury, drainace of intestine lympd fluid, dicd mobility croup box 1