Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica ›› 2009, Vol. 31 ›› Issue (4): 410-412.doi: 10.3881/j.issn.1000-503X.2009.04.005

• Original Articles • Previous Articles     Next Articles

Effect of Mycobacterium Smegmatis Vaccine on the Level of Nitric Oxide Produced by Peritoneal Macrophages in Mice

XU Miao; CHEN Bao-wen; SHEN Xiao-bing; SU Cheng; WANG Guo-zhi   

  1. 1st Bacterial Vaccine Division,National Institute for the Control of Pharmaceutical and Biological Products, Beijing 100050, China
  • Received:2009-03-31 Revised:1900-01-01 Online:2009-08-30 Published:2009-08-30
  • Contact: WANG Guo-zhi

Abstract: ABSTRACT:Objective To study the effect of Mycobacterium smegmatis vaccine on the level of nitric oxide (NO) produced by peritoneal macrophages in immunized mice. Methods Balb/c mice were randomized into low-dose, middle-dose, and high-dose groups (injected with different doses of Mycobacterium smegmatis vaccine) and a control group (injected with normal saline). Then the peritoneal macrophages were cultured with lipopolysaccharide in vitro. The supernatants were collected and the concentrations of NO were analyzed through the reaction with Griess reagents. Results The levels of NO produced by the peritoneal macrophages in the control group, low-dose group, middle-dose group, and high-dose group were (3.50±3.11) ,(16.63±6.47), (13.97±6.20),and (7.55±2.26) ng/ml, respectively. The levels of NO in all dosing groups were significantly different from that in control group (P<0.01). Conclusion Mycobacterium smegmatis vaccine can promote the peritoneal macrophages to produce NO in mice.

Key words: mycobacterium smecmatis, vaccine, nitric oxide