Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica ›› 2009, Vol. 31 ›› Issue (4): 413-416.doi: 10.3881/j.issn.1000-503X.2009.04.006

• Original Articles • Previous Articles     Next Articles

Establishment and Evaluation of Nitrate Reductase Combined with Mycobacteriophage Assay

XING Ai-ying; LIU Zhong-quan; JIA Hong-yan; GU Shu-xiang;ZHANG Zong-de   

  1. Laboratory of Molecular Biology for Tuberculosis, Beijing Tuberculosis and Thoracic
    Tumor Research Institute, Beijing 101149,China
  • Received:2009-02-12 Revised:1900-01-01 Online:2009-08-30 Published:2009-08-30
  • Contact: ZHANG Zong-de

Abstract: ABSTRACT:Objective To establish a rapid, inexpensive, and simple drug susceptibility test (DST ) for Mycobacterium tuberculosis (M.tb) and evaluate its feasibility. MethodWe used nitrate reductase combined with mycobacteriophage assay( PhaB-NRA) to test 49 clinical M.tb isolates of, and the results were compared with those of PhaB-NRA and traditional absolute concentration method. Results The sensitivity, specificity, and accuracy of PhaB-NRA for rifampicin were 89.1%, 91.67%, and 89.8%; on the contrary, those of isonicotinyl hydrazide were 86.21%, 90.0%, and 87.8%, respectively. The coincidence between PhaB-NRA and traditional assay were 0.746 for rifampicin and 0.750 for isonicotinyl hydrazide. Conclusions PhaB-NRA is an inexpensive, rapid, and simple DST method. It is a promising rapid screening technique for DST of M.tb.

Key words: mycobacterium tuberculosis, mycobacteriopdace, nitrate reductase, antituberculosis druc, druc susceptibility test