Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica ›› 2009, Vol. 31 ›› Issue (4): 449-452.doi: 10.3881/j.issn.1000-503X.2009.04.013

• Original Articles • Previous Articles     Next Articles

Sensitivity of Interferon-gamma Release Assay T-SPOT.TB in Diagnosing Extrapulmonary Tuberculosis

HUO Fei-fei;ZHANG Li-fan;LIU Xiao-qing   

  1. Department of Infectious Diseases, PUMC Hospital, CAMS and PUMC, Beijing 100730, China
  • Received:2009-05-19 Revised:1900-01-01 Online:2009-08-30 Published:2009-08-30
  • Contact: LIU Xiao-qing

Abstract: ABSTRACT:Objective To evaluate the sensitivity of an interferon-gamma release assay T-SPOT.TB in the diagnosis of bacteriologically or histologically confirmed extrapulmonary tuberculosis. MethodTotally 31 patients with bacteriologically or histologically confirmed extrapulmonary tuberculosis in Peking Union Medical College Hospital received T-SPOT.TB assay to detect early secreting antigen target 6 or culture filtrate protein 10 peptides-specific T cells in the peripheral blood mononuclear cells(PBMCs). Results T-SPOT.TB assay showed positive results in 29 patients with extrapulmonary tuberculosis and the sensitivity was 93.5%(95% CI 84.8% - 100%). The median of spot forming cells(SFCs) in response to early secreting antigen target 6 peptides was 196/106PBMCs (interquartile range, 72-532/106 PBMCs), the median of SFCs in response to culture filtrate protein 10 peptides was 276 SFCs/106 PBMCs (interquartile range, 72-568/106 PBMCs), and the median of the incorporate SFCs was 612/106PBMCs (interquartile range, 192-1152/106 PBMCs). Conclusion T-SPOT.TB is highly sensitive in diagnosing extrapulmonary tuberculosis.

Key words: extrapulmonary tuberculosis, sensitivity, t-spot.tb, early secretinc anticen tarcet 6 peptides, culture filtrate protein 10 peptides, interferon-camma release assay