Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica ›› 2009, Vol. 31 ›› Issue (4): 481-487.doi: 10.3881/j.issn.1000-503X.2009.04.020

• Original Articles • Previous Articles     Next Articles

Effect of Lewis y Antigen on Regulating Gene Expression of Partial Drug Resistance Associated Proteins in Human Ovarian Cancer Cell Line RMG-I-H

LIU Qing;LIN Bei;WANG Peng-li;YAN Li-mei;HAO Ying-ying;LI Fei-fei;ZHU Lian-cheng;ZHANG Shu-lan   

  1. Department of Obstetrics and Gynecology, Shengjing Hospital Affiliated to China Medical University, Shenyang 110004, China
  • Received:2008-10-09 Revised:1900-01-01 Online:2009-08-30 Published:2009-08-30
  • Contact: LIN Bei

Abstract: ABSTRACT:Objective To investigate the influence of Lewis y antigen on the gene expression of partial drug resistance associated proteins in human ovarian cancer cell line RMG-I-H. Methods RT-PCR was used to determine the gene expressions of partial drug resistance associated proteins in RMG-I-H cell line transfected with α1, 2-fucosyltransferases gene and RMG-I cell line, as well as in RMG-I-H treated with or without anti-Lewis y monoclonal antibody at the concentration of 10 μg/ml. The immunocytochemical method was used to detect the expression of P-glycoprotein (P-gp) in RMG-I and RMG-I-H cell lines. RMG-I and RMG-I-H cells were transplanted into nude mice and the expression of P-gp in the tissues was measured by immunohistochemistry. Results The mRNA expressions of protein kinase C-α(PKC-α), topoismeraseⅠ(Topo Ⅰ), multidrug resistance-associated protein-1(MRP-1), and MRP-2 were significantly higher in RMG-I-H cells than those in RMG-I cells (0.46±0.02 vs. 0.27±0.05, 0.82±0.08 vs. 0.52±0.04, 0.66±0.07 vs. 0.34±0.12, and 0.44±0.08 vs. 0.23±0.05; all P<0.05). However, the mRNA expression of multidrug resistance 1 (MDR-1) was significantly lower in RMG-I-H cells than that in RMG-I cells (0.26±0.05 vs. 0.45±0.08, P<0.05). The P-gp level increased in RMG-I-H cells compared with that in RMG-I cells both in vivo and in vitro (P<0.05). Expressions of MDR-1, MRP-1, MRP-2, PKC-α, and TopoⅠ mRNA decreased by the time in RMG-I-H cells treated with anti-Lewis y monoclonal antibody (all P<0.05), while mRNA expressions of those genes in the control group did not statistically change (P>0.05). In addition, MDR-1, MRP-1, MRP-2, PKC-α, and TopoⅠ mRNA expressions were significantly lower in RMG-I-H cells treated with anti-Lewis y monoclonal antibody than those in the control group at 6 hours (all P<0.05) and the inhibition ratios were 48.55%, 77.50%, 70.18%, 45.86%, and 46.13%, respectively. Conclusion The Lewis y antigen of the human ovarian cancer cell surface is closely correlated with the regulation on the gene expression of partial drug resistance associated proteins.

Key words: lewis y anticen, lewis y monoclonal antibody, ovarian cancer, druc resistance associated proteins