Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica ›› 2009, Vol. 31 ›› Issue (5): 522-526.doi: 10.3881/j.issn.1000-503X.2009.05.002

• Original Articles • Previous Articles     Next Articles

FLT3 Mutation in Patients with Acute Lymphoblastic Leukemia and Its Clinical Significance

ZHAO Xin;TANG Ke-jing;TIAN Zheng; CHEN Li-ping; MI Ying-chang;WANG Jian-xiang   

  1. State Key Laboratory of Experimental Hematology, Department of Hematologic Oncology,Institute of Hematology
  • Received:2009-04-30 Revised:1900-01-01 Online:2009-10-30 Published:2009-10-30
  • Contact: WANG Jian-xiang

Abstract: ABSTRACT:Objective To investigate fms-like tyrosine kinase 3 (FLT3) mutation in patients with acute lymphoblastic leukemia (ALL) and its clinical significance. Methods FLT3 mutation-internal tandem duplication (FLT3-ITD) and FLT3 mutation in the tyrosine kinase domain (FLT3-TKD) were detected using polymerase chain reaction (PCR) in the genomic DNA of 61 ALL patients and 7 healthy volunteers (as reference group). The PCR products of these patients who were detected with FLT3-ITD or FLT3-TKD were sent for sequence analysis. Results In all these 61 ALL patients,FLT3-ITDs were identified in 2 patients and FLT3-TKD were identified in one patient. Conclusion FLT3-ITD and FLT3-TKD exist in a small proportion of ALL patients.

Key words: flt3 cene, flt3 mutation-internal tandem duplication, flt3 mutation in tde tyrosine kinase domain, acute lympdoblastic leukemia