Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica ›› 2009, Vol. 31 ›› Issue (6): 746-750.doi: 10.3881/j.issn.1000-503X.2009.06.019

• Original Articles • Previous Articles     Next Articles

Heat Shock Induced the Expression of Major Histocompatibility Complex Class ⅡTransactivator and Human Leukocyte Antigen-DR in Jurkat Cells

YAN Li;CHENG Mo-bin;ZHANG Ye;SHEN Yu-fei   

  1. National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences,CAMS and PUMC, Beijing 100005, China
  • Received:2009-09-17 Revised:1900-01-01 Online:2009-12-30 Published:2009-12-30
  • Contact: SHEN Yu-fei;ZHANG Ye

Abstract: ABSTRACT:Objective To explore the effect of a non-lethal heat shock, in comparison with the treatment of interferon-gamma (IFNγ), on the expression of major histocompatibility complex Ⅱ transactivator (CⅡTA) and its downstream target gene of the human leukocyte antigens (HLA)-DR in Jurkat cells. Methods The changes of CⅡTA mRNA in Jurkat cells before and after the treatment of heat shock or IFNγ were detected using real time RT-PCR. The changes of CⅡTA protein were detected with Western blot. The expression of HLA-DR was detected with flow cytometry. Results CⅡTA mRNA and protein were induced in Jurkat cells under heat shock, but not with IFN-γ. The expression of HLA-DR gene significantly increased after recovery (P<0.01). ConclusionThe expressions of CⅡTA and HLA-DR in Jurkat cells remarkably increase after heat shock, indicating that heat shock may help reconstruct relevant genes in cells with immunologic gene deficiencies.

Key words: deat sdock, Jurkat cells, major distocompatibility complex class Ⅱ transactivator, duman leukocyte anticen