Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica ›› 2011, Vol. 33 ›› Issue (1): 71-75.doi: 10.3881/j.issn.1000-503X.2011.01.015

• Original Articles • Previous Articles     Next Articles

Influence of Tumor associated Macrophages on Biological Function of SW620 Cell

ZHANG Yin xu1, LIU Xiao mei2, ZUO Teng1, LIU Yu1, ZHANG Jun hua1   

  1. 1Department of General Surgery,2Department of Medical Oncology, the First Affiliated Hospital of Liaoning Medical University, Jinzhou, Liaoning 121001, China
  • Received:2010-11-12 Revised:2011-03-05 Online:2011-02-10 Published:2011-02-10
  • Contact: 辽宁省教育厅重点实验室项目(LS2010102)

Abstract: Objective To study the influence of tumor-associated macrophages (TAMs) on the biological function of SW620 cell. Methods Macrophage was induced into M2-type macrophage form with interleukin (IL)-4. CD68, macrophage mannose receptor (MMR), and inducible nitric oxide synthase (iNOS) were analyzed with Western blot. SW620 was co-cultured with TAMs in the Transwell. Cytokines including IL-10, IL-12, IL-23, and tramsforming growth factor-β(TGF-β) were detected with enzyme-linked immunosorbent assay (ELISA). The activity of nuclear factor-κB (NF-κB) in SW620 was analyzed with electrophoretic mobility shift assay (EMSA). The proliferation and apoptosis of SW620 cells after co-cultured with TAM were determined with tetrazolium four nitrogen (XTT) assay and fluorescence activated cell sorting (FACS), respectively. Results IL-4 induced M2 type macrophage expressed CD68 and MMR instead of iNOS. After co-cultured with SW620 for 24 hours and 48 hours, M2 type macrophage secreted higher levels of IL-10 and TGF-β than the pre-culture level (P<0.01), although IL-12 and IL-23 showed no significant differences (P>0.05). The activity of NF-κB in SW620 decreased by 72% and 75% after 24 hours and 48 hours compared with the pre-culture level, respectively (both P<0.01). The activity of proliferation decreased by 48% and 59% and the apoptotic rates increased by 6.37% and 7.68% and 0.37% after 24 hours and 48 hours (all P<0.01) compared with the pre-culture levels. Conclusion TAM may inhibit the proliferation and promote the apoptosis of SW620 by suppressing the activity of NF-κB.

Key words: tumor-associated macrophages, SW620 cells, co-culture, proliferation, apoptosis

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