Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica ›› 2011, Vol. 33 ›› Issue (4): 382-386.doi: 10.3881/j.issn.1000-503X.2011.04.007

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Biological Features and Ultrastructure of Human Umbilical Cord Mesenchymal Stem Cells

ZHU Shaofang, HE Yuanli, FU Xiafei   

  1. Department of Obstetrics and Gynecology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282,China
  • Received:2010-12-17 Revised:2011-09-05 Online:2011-08-01 Published:2011-08-01
  • Contact: HE Yuan-li
  • Supported by:
    Supported by the fund of research project of Guangdong Province(B2010203)

Abstract: Objective To isolate and culture human umbilical cord mesenchymal stem cells (MSCs) and explore their biological features and ultrastructure. Methods After isolating MSCs from the human umbilical cord, the proliferation, cycle, and apoptosis were observed. The cell ultrastructure was observed under transmission electron microscope. The cytokines including vascular endothelial growth factor (VEGF), hepatocyte growth factor (HGF), and insulin-like growth factor-1 (IGF-1) were detected using enzyme-linked immunosorbent assay. Results Human umbilical cord MSCs had fibroblast-like morphology and increased proliferation capability. Ultrastructural analysis showed that the MSCs had active cellular metabolism and strong migration and differentiation capabilities. Meanwhile, they could secrete anti-apoptotic cytokines such as VEGF, IGF-1, and HGF.Conclusion Human umbilical cord MSCs can secrete many anti-apoptotic cytokine and have good biological features.

Key words: human umbilical cord, mesenchymal stem cell, ultrastructure

CLC Number: