Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica ›› 2011, Vol. 33 ›› Issue (5): 538-542.doi: 10.3881/j.issn.1000-503X.2011.05.012

• Original Articles • Previous Articles     Next Articles

Growth of Osteoblasts Following Culture on Two Collagen Scaffolds

MA Chao, ZHANG Ding, LI Ping, LI Xiao-jiao   

  1. Department of Stomatology, PUMC Hospital, CAMS and PUMC, Beijing 100730, China
  • Received:2011-01-25 Revised:2011-10-28 Online:2011-10-28 Published:2011-10-28
  • Contact: ZHANG Ding E-mail:dingz77@sina.com
  • Supported by:

    Supported by the National Natural Sciences Foundation of China(31070829)

Abstract: Objective To explore the growth of osteoblasts following culture on two absorbable collagen scaffolds. Methods MC3T3-E1 osteoblast cell line was inoculated on two collagen scaffolds (BME-10X® and Bio-Gide®) and co-cultured in vitro. The adhesion and migration of cells were detected by optical microscope. Cell proliferation was detected using CCK-8 reagent kit. The ultrastructure of the adhesion between cells and scaffolds were observed using electronic microscopy. Results MC3T3-E1 osteoblast cell line could adhere, migrate, and proliferate on both two membranes. The proliferation of cells showed no significant difference between porous layer and nonporous layer of BME-10X® (P>0.05),while the rate of proliferation was significantly different between loose layer and dense layer of Bio-Gide® (P<0.05). Conclusion Both BME-10X® and Bio-Gide® have good biocompatibility with MC3T3-E1 osteoblast cell line, and the double-layer structure of Bio-Gide® can prevent the cells to grow into the dense layer.

Key words: osteoblasts, biocompatibility, guided tissue regeneration, periodontal tissue engineering

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