Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica ›› 2011, Vol. 33 ›› Issue (6): 615-619.doi: 10.3881/j.issn.1000-503X.2011.06.006

• Original Articles • Previous Articles     Next Articles

Role of Extracellular Signal-regulated Protein Kinase 5 in the Biosynthesis of Follicle-stimulating Hormone-stimulated Progesterone in Primary Granulosa Cells

GAO Xiao-bo1, 2, YAO Nan3, MA Xu2, LU Cai-ling2, YANG Bo-qing2, PENG Xiao-zhong1   

  1. 1National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 100005, China; 2Department of Genetics, National Research Institute for Family Planning, Beijing 100081, China; 3Office of Primary Health Care and Maternal Child Health Care Research, Institute of Medical Information,CAMS and PUMC, Beijing 100020, China
  • Received:2011-10-20 Revised:2012-01-04 Online:2011-12-20 Published:2011-12-20
  • Contact: PENG Xiao-zhong
  • Supported by:

    Supported by the National Basic Research Program of China (973 Program) (2010CB529504) and the Central Publicinterest Scientific Institution Basal Research Fund (2009GJSSJKA07)

Abstract: Objective To study the role of extracellular signal-regulated protein kinase 5 (ERK5) during the biosynthesis of follicle-stimulating hormone (FSH)-mediated progesterone in primary granulosa cells. MethodsThe expressions of phophorylated and general forms of ERK5 in primary granulosa cells after the treatment of FSH were detected by Western blot analysis. The subcellular localization of ERK5 was observed by confocal microscopy. The effect of ERK5 on FSH-mediated progesterone biosynthesis in primary granulosa cells was analyzed using recombinant adenovirus vectors. ResultsERK5 activation was induced by FSH in a time-dependent manner in primary cultured granulosa cells, although the general ERK5 protein level decreased also in a time-dependent manner. The treatment of FSH showed no remarkable effect on the subcellular distribution of endogenous ERK5, which was mainly in the cytoplasm of granulosa cells. The co-infection of Ad-caMEK5 and Ad-wtERK5 increased the progesterone production and StAR expression in primary cultured granulosa cells, whereas inhibition of ERK5 activation suppressed the FSH-stimulated progesterone production. ConclusionERK5 may stimulate FSH-mediated progesterone production in primary cultured granulosa cells.

Key words: extracellular signal-regulated protein kinase 5, follicle- stimulating hormone, granulosa cells, steroid hormones, progesterone

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