Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica ›› 2011, Vol. 33 ›› Issue (6): 629-631.doi: 10.3881/j.issn.1000-503X.2011.06.009

• Original Articles • Previous Articles     Next Articles

Separation and Morphological Observation of Mouse Testis Seminiferous Tubule Epithelium

FU Jun, MIAO Shi-ying   

  1. National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences,CAMS and PUMC, Beijing 100005, China
  • Received:2011-10-18 Revised:2012-01-04 Online:2011-12-20 Published:2011-12-20
  • Contact: MIAO Shi-ying E-mail:miao.shiying@imicams.ac.cn
  • Supported by:

    Supported by the National Important Research Plan of China(2011CB944302) and the State Key Laboratory Special fund (2060204)

Abstract: Objective To explore a simple and feasible technique to locate proteins during spermatogenesis. Methods Various germ cells and somatic cells were separated by collagenase I and DNase I after the albuginea was removed. The cells were then smeared, dyed, and observed directly under fluorescence and confocal microscopy. Results Germ cells at different steps were successfully identified by specific dyestuffs for acrosome and nucleolus. Conclusion A simple method for locating proteins during permatogenesis was successfully developed.

Key words: spermatogenesis, acrosome formation, nucleolus

CLC Number: