Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica ›› 2012, Vol. 34 ›› Issue (1): 1-7.doi: 10.3881/j.issn.1000-503X.2012.01.001

• Original Articles •     Next Articles

Effects of a Highcarbohydrate Diet on the Serum Lipid and Apolipoprotein Ratios in Healthy Young Adults with Different Genotypes of APOA1-75 G/A Polymorphism

SONG Yong-yan1,2, OU Guo-jin1, GONG Ren-rong3, ZHANG Zhen1, HU Min-shan1, FAN Mei1, LI Yuan-hao1, FANG Ding-zhi1   

  1. 1Department of Biochemistry and Molecular Biology Laboratory, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China; 2Department of Biochemistry, School of Preclinical Medicine, North Sichuan Medical College,Nanchong, Sichuan 637007, China; 3Department of Cardiac and Thoracic Surgery, West China Hospital, Sichuan University, Chengdu 610041, China
  • Received:2011-05-10 Revised:2012-03-02 Online:2012-03-01 Published:2012-03-01
  • Supported by:

    Supported by the Program for New Century Excellent Talents in Universities in China (NCET-04-0863)

Abstract: Objective To investigate the effects of a high-carbohydrate diet on the lipid and apolipoprotein ratios in healthy young adults with different genotypes of the polymorphism at -75 site in the promoter region of the gene of apolipoprotein AI (APOA1). Methods Fifty-six subjects aged (22.89?1.80) years were given a wash-out diet for 7 days, followed by a high-carbohydrate diet for 6 days. The wash-out diet contained 15% protein, 31% fat, and 54% carbohydrate. The high-carbohydrate diet contained 15% protein, 15% fat, and 70% carbohydrate. Twelve-hour fasting serum lipids and apolipoproteins B100 and AI were measured on the mornings of the 1st, the 8th, and the 14th days from the beginning of the wash-out diet. The ratios of triglyceride (TG)/high-density lipoprotein cholesterol (HDL-C), total cholesterol (TC)/HDL-C, low-density lipoprotein cholesterol (LDL-C)/HDL-C, and apolipoprotein B100 (APOB100)/apolipoprotein AI (APOAI) were calculated. The genome DNA was extracted and the polymorphism of APOA1-75 G/A was determined by polymerase chain reaction followed by restriction fragment length polymorphism assay. Results At baseline, the lipid and apolipoprotein ratios showed no significant differences between the GG genotype and the A carriers in males (P>0.05), whereas the female A carriers had a significantly higher ratio of LDL-C/HDL-C compared with the female subjects with the GG genotype (P<0.05). Following the high-carbohydrate diet, significant decreases of TC/HDL-C were found in all the groups, regardless of sex and genotype (P<0.01). LDL-C/HDL-C experienced significant decreases in both the genotypes in males (P<0.05), while in females, significant decrease of LDL-C/HDL-C was only observed in A carriers (P<0.01). Conclusion The A allele of the -75 G/A polymorphism in APOA1 may have specific effects on the LDL-C/HDL-C ratio in females.

Key words: apolipoprotein AI, gene polymorphism, high-carbohydrate diet, lipid and apolipoprotein ratios

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