Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica ›› 2012, Vol. 34 ›› Issue (1): 14-18.doi: 10.3881/j.issn.1000-503X.2012.01.003

• Original Articles • Previous Articles     Next Articles

Effects of Different Hepatic Inflow Occlusion Methods on LiverRegeneration Following Partial Hepatectomy in Rats

WANG Peng-fei, LI Chong-hui, ZHANG Ai-qun, CAI Shou-wang, DONG Jia-hong   

  1. Department of Hepatobiliary Surgery and Hepatobiliary Surgical Institute, Chinese PLA General Hospital, Beijing 100853, China
  • Received:2011-06-27 Revised:2012-03-02 Online:2012-03-01 Published:2012-03-01
  • Supported by:

    Supported by the Major National Science and Technology Project (2008ZX10002-026)

Abstract: Objective To explore the effects of different hepatic inflow occlusion methods on liver regeneration in rats after partial hepatectomy (PH). Methods Male Wistar-Furth rats were randomly assigned to three groups: control group, underwent 68% hepatectomy alone; occlusion of portal triad (OPT) group, subjected to occlusion of portal triad under portal blood bypass; and occlusion of portal vein (OPV) group, subjected to occlusion of portal vein under portal blood bypass. Blood flow was occluded for 20, 30, and 40 minutes before 68% hepatectomy. According to the 7-day survival of each group, a same occlusion time T was set. Each group was divided into two subgroups (n=8), in which animals were humanly killed 3 and 7 days later. Liver regeneration was calculated as a percent of initial liver weight. Immunohistochemistry for proliferating cell nuclear antigen (PCNA) and Ki-67 was performed to quantify proliferating cells. In addition, functional liver volume represented by 99Tcm-GSA radioactivity was assessed. Results The safe tolerance limit time was 30 minutes for OPT group and 40 minutes for OPV group. At 3 days after PH, no significant difference was observed in the regeneration rate of each group (P>0.05). However, liver radioactive activity, PCNA labeling index, and Ki-67 index of OPV group was significantly higher than those of OPT group (P0.05). At 7 days after PH, no significant difference was observed in all indexes among three groups (P>0.05). Conclusion Compared with Pringle maneuver, preserving the hepatic artery flow during portal triad blood inflow occlusion can promote remnant liver regeneration early after PH.

Key words: hepatectomy, hepatic inflow occlusion, liver regeneration, radioactive activity, rat

CLC Number: