Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica ›› 2012, Vol. 34 ›› Issue (3): 212-215.doi: 10.3881/j.issn.1000-503X.2012.03.004

• Original Articles • Previous Articles     Next Articles

Expression of Gene Associated with Retinoid-interferon-induced Mortality-19 in Preimplantation Embryo of Mice

CUI Wen-juan, CHAO Lan, DENG Xiao-hui, SHEN Yan-jun, YANG Fang, FENG Wen-juan, XU Jing, CHEN Hong-lei   

  1. Infertility Center, Qilu Hospital, Shandong University, Jinan 250012, China
  • Received:2011-11-16 Revised:2012-06-30 Online:2012-06-29 Published:2012-06-29
  • Supported by:

    Supported by the National Natural Sciences Foundation of China(30901603),the Research Award Fund for Excellent Young Scientist of Shandong Province(BS2009SW031),and the Shandong Provincial Family Planning Commission Science and Technology Funds(2009 NO.8)

Abstract: Objective To study the expression of gene associated with retinoid-interferon-induced mortality-19(GRIM-19) in preimplantation embryo of mice and explore its role in embryonic development. Methods The protein and mRNA expressions of GRIM-19 in 2-cell, 4-cell, 8-cell, morula, and blastocyst phases of mice preimplantation embryo were detected by Western blot analysis and Real-time polymerase chain reaction (PCR). Results GRIM-19 was continuously expressed in every stage of preimplantation embryo of mice. Western blot analysis and Real-time PCR demonstrated a gradual increase of GRIM-19 expression from 2-cell, which reached a peak in 8-cell phase and then decreased progressively. Conclusions The expression of GRIM-19 in mouse preimplantation embryos changes as at different developmental phases.GRIM-19 may play an important role during embryonic development.

Key words: gene associated with retinoid-interferon-induced mortality-19, preimplantation embryo, mitochondria

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