Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica ›› 2012, Vol. 34 ›› Issue (3): 216-221.doi: 10.3881/j.issn.1000-503X.2012.03.005

• Original Articles • Previous Articles     Next Articles

Effect of Electroconvulsive Shock on the Glutamate Level and the Hyperphosphorylation of Protein Tau in Depression Rat Models Whose Olfactory Bulbs Were Removed

LIU Chao1, MIN Su1, WEI Ke1, LIU Dong2, DONG Jun1, LUO Jie1, LIU Xiao-Bin1   

  1. 1Department of Anesthesiology, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China
    2Department of Neurosurgery, the Second Hospital of Tianjin Medical University, Tianjin 300211, China
  • Received:2011-11-11 Revised:2012-06-30 Online:2012-06-29 Published:2012-06-29
  • Supported by:

    Supported by the National Natural Sciences Foundation of China (30972831)

Abstract: Objective To explore the effect of the electroconvulsive shock (ECS) on the glutamate level and the hyperphosphorylation of tau protein in depressed rats. Methods The depression rat models whose olfactory bulbs were removed were established. Using the analysis of variance of factorial design, we set up two intervention factors including electric current (three levels: 25, 50, and 75 mA) and duration (three levels: 3, 6, and 9 times), which constituted 9 combinations (n=6). Fifty-four adult depression rat models whose olfactory bulbs were removed were randomly divided into nine experimental groups (n=6 in each group). The hippocampus was removed within 12 hours after the ECS finished. The level of glutamate in the hippocampus was detected by high-performance liquid chromatography, and that of Tau protein, which includes p-PHF-1Ser396/404, p-AT8Ser199/202, and p-12E8Ser262, in the hippocampus with Western blot analysis. Results The glutamate level and the hyperphosphorylation of tau protein in the hippocampus of depressed rats remarkably increased. The changes of the hyperphosphorylation of tau protein were correlated with the electric current and duration of ECS, and these two factors showed an synergic effect. Conclusion ECS enhances the hyperphosphorylation of tau protein in the hippocampus of depressed rats by up-regulating the glutamate level.

Key words: electroconvulsive therapy, depression, tau protein, hyperphosphorylation, glutamic acid

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