Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica ›› 2012, Vol. 34 ›› Issue (3): 244-248.doi: 10.3881/j.issn.1000-503X.2012.03.010

• Original Articles • Previous Articles     Next Articles

Identification and Expression of A Tumor-associated Antigen in Esophageal Squamous Cell Carcinoma

GAO Hong-jun1, YUE Zhi-gang1, ZHENG Min1, ZHENG Zhao-xu2   

  1. 1Clinical Laboratory, Coal General Hospital, Beijing 100028, China
    2Department of Abdominal Surgery, Cancer Institute & Hospital, CAMS and PUMC, Beijing 100021, China
  • Received:2012-02-15 Revised:2012-06-30 Online:2012-06-29 Published:2012-06-29

Abstract: Objective To search for novel tumor associated antigens (TAA) in esophageal squamous cell carcinoma (ESCC). Methods The proteins extracted from tissues of ESCC were separated by two dimensional polyacrylamide gel electrophoresis and transferred to PVDF membrane. Sera from ESCC patients and healthy individuals were used for primary antibodies for Western blot analysis. The differential spots were excised for trypsin hydrolysis and the tryptic peptides were analyzed by matrix-assisted laser disorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). The identified TAA of ESCC was validated by immunohistochemical staining (IHC). Results Sera from ESCC patients yielded multiple positive spots, and one 28 800 Da protein that exhibited positive reactivity with 60% (12/20) sera of ESCC patients and only 5% (1/20) sera of healthy controls (P<0.01). The 28 800 Da protein was identified as phosphoglycerate mutase 1 (PGAM1) by MALDI-TOF-MS. Immunohistochemical analysis showed that PGAM1 was located in both cytoplasm and nucleus, and had a higher expression in cancer tissues. Conclusion PGAM1 maybe a candidate of ESCC.

Key words: tumor associated antigen, esophageal squamous cell carcinoma, phosphoglycerate mutase 1, expression, identificaton

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