Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica ›› 2013, Vol. 35 ›› Issue (3): 254-259.doi: 10.3881/j.issn.1000-503X.2013.03.003

• Orignal Article • Previous Articles     Next Articles

Role of HPV16/18/45 DNA Testing of Cervical Specimens as A Triage Testing in Cervical Cancer Screening

LI Jing1,CHEN Wen1,KANG Le-ni1,CHEN Feng 1,GUO Dong-ping 2, LI Ba-yi2,QIAO You-lin1   

  1. 1Department of Cancer Epidemiology,Cancer Institute & Hospital,CAMS and PUMC,Beijing 100021,China
    2 Medical Department,Yangcheng Women & Children’s Hospital,Yangcheng,Shanxi 048100,China
  • Received:2013-03-28 Online:2013-07-04 Published:2013-07-04
  • Contact: QIAO You-lin Tel:010-87788489,Fax:010-67713648,


Objective To evaluate the clinical performance of careHPV16/18/45 DNA testing of cervical specimens as a triage testing for women with positive findings during the cervical cancer screening.Methods Eligible women aged 25-65 years were enrolled from two high-risk communities in Yangcheng County,Shanxi Province.After providing written informed consent on a voluntary base,women underwent questionnaire-based interview,gynecological examination,and sample collection.Hybrid capture 2 technology(HC2),careHPV,Avantage HPV E6 test,and visual inspection with acetic acid(VIA)were conducted as the primary screening tests at the enrollment visit.Women with any positive finding were invited to receive a second VIA and colposcopy.careHPV16/18/45 was performed as a triage testing.Any visible lesion under colposcopy was directly biopsied.Women with pathology confirmed cervical intraepithelial neoplasia grade 2 and worse(CIN2+)were treated with standard procedures.Results For the self-collected and doctor-collected samples,the application of careHPV16/18/45 as a triage testing decreased the colposcopy referral to 3.2% and 3.1%,respectively.Meanwhile,the sensitivity,specificity,and positive predictive value(PPV)for CIN2+were 50.0%,97.6%,and 26.7% for women with positive self-sampling careHPV results and 63.0%,97.9%,and 34.0% for women with positive doctor-sampling careHPV results.Conclusion careHPV16/18/45 is promising as a triage testing among women with positive screening findings in low-resource settings.

Key words: careHPV16/18/45, cervical cancer, primary screening, positive, triage