Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica ›› 2013, Vol. 35 ›› Issue (4): 404-410.doi: 10.3881/j.issn.1000-503X.2013.04.009

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Effecacy of Cyclosporine A-nanoparticles Emulsion Combined with Stem Cell Transplantation Therapy for Acute Myocardial Infarction

YIN Qiao-xiang1,WANG Heng2,PEI Zhi-yong3,ZHAO Yu-sheng4   

  1. 1 Department of Geriatric Cardiology, 2 Department of Neurology,Chinese General Hospital of Air Force,Beijing 100142,China
    3 Department of Geriatric Cardiology,Chinese General Hospital of Beijing Military Region,Beijing 100700,China
    4 Institute of Geriatric Cardiology,Chinese PLA General Hospital,Beijing 100853,China
  • Received:2012-11-27 Online:2013-08-30 Published:2013-08-30
  • Supported by:

    Supported by the National High Technology Research and Development Program of China(863 Program)(2006AA02A105)

Abstract:

Objective To evaluate the efficacy of cyclosporine A-nanoparticles emulsion(CsA-NP)combined with adipose tissue-derived stem cells(ASCs)transplantation therapy for acute myocardial infarction(AMI)in a miniswine model.Methods CsA-NP emulsion was prepared by the high-pressure homogenization method.Models were performed by coronary angioplasty for percutaneous balloon occlusion of left anterior descending artery(LAD).A total of 17 miniswines survived after AMI were divided into four groups:control group(n=5),CsA-NP group(n=4),ASCs group(n=4),and CsA-NP+ASCs group(n=4).ASCs or saline were delivered by intracoronary injection one week after AMI.Before cell transplantation and 8 weeks after cell transplantation,delayed-enhanced magnetic resonance imaging(DE-MRI)was performed to evaluate cardiac function and viability.The infarcted myocardium and implanted cells were histologically studied.Results Eight weeks after treatment,the left ventricular ejection fraction(LVEF)significantly increased in the CsA-NP+ASCs group when compared with the ASCs group[(53.6±2.4)% vs.(48.3±1.8)%,P<0.05];meanwhile,the infarct size significantly decreased[(6.2±1.7)cm3 vs.(7.5±0.6)cm3,P<0.05]and the thickness of the ventricular wall significantly increased(P<0.05).Histology showed that the number of surviving cells increased nearly by three times in the CsA-NP+ASCs group,and the expressions of the cardiomyocyte specific markers(cTnT and α-actin)were detected.Histological samples also showed that CsA-NP+ASCs group reduced fibrotic tissue,and down-regulated the activation of Caspase-3.Conclusion The CsA-NP+ASCs combination therapy can enhance the viability of ASCs by improving LVEF and preventing LV expansion,which may be explained that CsA-NP has the anti-apoptotic effect and can promote the survivals and proliferation of ASCs.

Key words: cyclosporine A, nanoparticles, stem cell, adipose, apoptosis, acute myocardial infarction