Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica ›› 2013, Vol. 35 ›› Issue (4): 432-438.doi: 10.3881/j.issn.1000-503X.2013.04.014

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Mechanisms of Icariin in Regulating Bone Formation of Osteoblastsand Bone Resorption of Osteoclasts

MA Xiao-ni,GE Bao-feng,CHEN Ke-ming,ZHOU Jian,SHI Wen-gui,XIE Yan-fang,GUO Xiao-yu,L Xiang,CHENG Kui,GAO Yu-hai   

  1. Institute of Orthopaedics,Lanzhou General Hospital,Lanzhou Command of PLA,Lanzhou 730050,China
  • Received:2012-07-03 Online:2013-08-30 Published:2013-08-30
  • Contact: CHEN Ke-ming Tel:09318994327,
  • Supported by:
    Supported by the Major Science and Technology Project of Gansu Province(09ZNKDA025)

Abstract: Objective To investigate the molecular mechanisms of icariin(ICA)in regulating the bone formation of osteoblasts and the bone resorption of osteoclasts.Methods Primary osteoblast cell cultures were obtained from newborn rat calvarial.Calcified nodules were stained by alizarin red.The mRNA levels of osterix(OSX),runt-related transcription factor 2(Runx-2),alkaline phosphatase(ALP),CollagenⅠ,osteoprotegerin(OPG),and receptor activator of nuclear factor-κB ligand(RANKL)were analyzed by quantitative real-time RT-PCR,the protein levels of OPG,RANKL,and CollagenⅠ were examined by Western blotting,and the intracellular Ca2+concentration of osteoblasts was measured on a flow cytometer using the Cellquest program.Results Compared with control group,ICA markedly promoted bone formation by significant up-regulating the gene expressions of OSX,Runx-2,ALP,and CollagenⅠ,the protein expression of CollagenⅠ(all P<0.01),and the Ca2+concentration.Furthermore,ICA remarkably inhibited bone resorption by significant up-regulating the mRNA and protein expressions of OPG as well as the OPG/RANKL ratio.Conclusions ICA could promote bone formation of osteoblasts through inducting the gene expressions of OSX,Runx-2,ALP and CollagenⅠ,and the protein expressions of CollagenⅠ,and by increasing the Ca2+concentration.Moreover,ICA could inhibit bone resorption of osteoclasts through regulating OPG/RANKL signal pathway.

Key words: osteoblasts, receptor activator of nuclear factor-κB ligand, osteoprotegerin, icariin, flow cytometry