Acta Academiae Medicinae Sinica

Abstract:

Objective To establish a mouse model of iron overload by intraperitoneal injection of iron dextran and investigate the impact of iron overload on bone marrow hematopoiesis.Methods A total of 40 C57BL/6 mice were divided into control group,low-dose iron group(12.5 mg/ml),middle-dose iron group(25 mg/ml),and high-dose iron group(50 mg/ml).The control group received normal saline(0.2 ml),and the rest were injected with intraperitoneal iron dextran every three days for six weeks.Iron overload was confirmed by observing the bone marrow,hepatic,and splenic iron deposits and the bone marrow labile iron pool.In addition,peripheral blood and bone marrow mononuclear cells were counted and the hematopoietic function was assessed.Results Iron deposits in bone marrow,liver,and spleen were markedly increased in the mouse models.Bone marrow iron was deposited mostly within the matrix with no significant difference in expression of labile iron pool.Compared with control group,the ability of hematopoietic colony-forming in three interventional groups were decreased significantly(P＜0.05).Bone marrow mononuclear cells counts showed no significant difference.The amounts of peripheral blood cells(white blood cells,red blood cells,platelets,and hemoglobin)in different iron groups showed no significant difference among these groups;although the platelets were decreased slightly in low-dose iron group[(780.7±39.60)×10⁹/L],middle dose iron group[(676.2±21.43)×10⁹/L],and high-dose iron group[(587.3±19.67)×10⁹/L]when compared with the control group[(926.0±28.23)×10⁹/L],there was no significant difference(P>0.05).Conclusions The iron-overloaded mouse model was successfully established by intraperitoneal administration of iron dextran.Iron overload can damage the hepatic,splenic,and bone marrow hematopoietic function,although no significant difference was observed in peripheral blood count.

Key words: iron overload, animal models, bone marrow hematopoietic functions