Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica ›› 2014, Vol. 36 ›› Issue (1): 1-4.doi: 10.3881/j.issn.1000-503X.2014.01.001

• Original Articles •     Next Articles

Identification of Proteins Interacted with Bat3 Using Tandem Affinity Purification

WU Wei, LI Qin-shan, SONG Wei, MIAO Shi-ying, WANG Lin-fang   

  1. National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 100005, China
  • Received:2013-05-17 Online:2014-02-28 Published:2014-02-28
  • Contact: WANG Lin-fang Tel:010-69156418,E-mail:wang.linfang@imicams.ac.cn E-mail:wang.linfang@imicams.ac.cn
  • Supported by:
    Supported by the National Important Research Plan of China(2011CB944302)and the State Key Laboratory Special Fund of China(2060204)

Abstract: Objective To identify the specific protein interactions involved in Bat3-mediated apoptosis.Methods Tandem affinity purification(TAP)was utilized to investigate Bat3-protein interactions,during which full-length human Bat3 fused with Strep2 and FLAG tag as a bait was used to screen the specific protein-protein interactions.The isolated proteins were identified with mass spectrometry.Results TAP studies showed that Ubl4A was identified as a Bat3-binding partner.Further investigation using co-immunoprecipitation confirmed that Bat3 was associated with Ubl4A.Conclusion TAP was successfully established and is suitable for isolating the binding partners of Bat3.

Key words: Bat3, apoptosis, tandem affinity purification

CLC Number: