斯钙素蛋白-1和缺氧诱导因子-1α的相互作用对肾癌细胞线粒体膜电势稳定的影响

doi:10.3881/j.issn.1000-503X.2014.01.003

Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica ›› 2014, Vol. 36 ›› Issue (1): 12-19.doi: 10.3881/j.issn.1000-503X.2014.01.003

• Original Articles • Previous Articles Next Articles

Effects of Stanniocalcin-1 and Hypoxia-inducible Factor-1α on Mitochondrial Membrane Potential Stability in Renal Carcinoma Cells

YANG Qing-tao¹, GU Jiang¹, ZHANG Yong-chun¹, ZHU Zhi-hui¹, YANG Yong-an¹, WANG Nan¹, ZHU Qing-liang²

¹Department of Urologic Surgery, Affiliated Hospital, Guiyang Medical College, Guiyang 550004, China ²Department of Urologic Surgery, Jiangdu People’s Hospital, Yangzhou, Jiangsu 225200, China

Received:2013-08-23 Online:2014-02-28 Published:2014-02-28
Contact: GU Jiang Tel:0851-6773096,E-mail:gj0851@gmail.com E-mail:gj0851@gmail.com
Supported by:
Supported by the Guizhou Province Science and Technology Department of Social Research Projects(黔科合SY字[2011]3060)

Abstract

Abstract:

Objective To explore the effects of stanniocalcin-1(STC-1)and hypoxia-inducible factor-1α(HIF-1α)on the calcium and thus on the mitochondrial membrane potential(Δψm)in renal carcinoma cells.Methods We successfully established the renal carcinoma cell models with high HIF-1α gene expression.After various concentrations of STC-1 solutions were added to the culture medium,the proliferation of cells,expressions of HIF-1α and STC-1,levels of Ca²⁺,Δψm,and mPTP were detected by MTT,RT-PCR,ELISA,fluorescence spectrophotometry,and ultraviolet spectrophotometry,respectively.Results The proliferation of renal carcinoma cells and Δψm were improved after HIF-1α gene transfection,STC-1 protein intervention,and STC-1 protein intervention after gene transfection.While the intracellular Ca²⁺level and mPTP were decreased significantly(P＜0.05),all the changes were intensified with the gradual increase of STC-1.However,the increasing trend of cell proliferation gradually declined.Conclusion HIF-1α may participate in malignant proliferation of renal carcinoma cells by promoting STC-1 proliferation or down-regulating Ca²⁺;however,such an effect may be gradually attenuated due to the inhibitory effect of STC-1 on HIF-1α.

Key words: renal carcinoma cells, stanniocalcin-1, hypoxia-inducible factor-1α, mitochondria

CLC Number:

R445.2

YANG Qing-tao, GU Jiang, ZHANG Yong-chun, ZHU Zhi-hui, YANG Yong-an, WANG Nan, ZHU Qing-liang. Effects of Stanniocalcin-1 and Hypoxia-inducible Factor-1α on Mitochondrial Membrane Potential Stability in Renal Carcinoma Cells[J].Acta Academiae Medicinae Sinica, 2014, 36(1): 12-19.

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Effects of Stanniocalcin-1 and Hypoxia-inducible Factor-1α on Mitochondrial Membrane Potential Stability in Renal Carcinoma Cells

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