宫颈鳞状细胞癌化疗前后小分子RNA-96的表达变化及其与化疗敏感性的关系

doi:10.3881/j.issn.1000-503X.2014.02.005

Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica ›› 2014, Vol. 36 ›› Issue (2): 140-144.doi: 10.3881/j.issn.1000-503X.2014.02.005

• Original Articles • Previous Articles Next Articles

Expressions of MicroRNA-96 Before and After Chemotherapy and Its Relationship with Chemosensitivity in Cervical Squamous Cell Carcinoma

ZHOU Wen,GUAN Ting

Department of Obstetrics and Gynecology,General Hospital of Guangzhou Military Command,Guangzhou 510010,China

Received:2013-08-23 Online:2014-04-28 Published:2014-04-28
Contact: GUAN Ting Tel:020-88653527,E-mail:lxgdut@163.com E-mail:lxgdut@163.com

Abstract

Abstract:

Objective To investigate the expression profiles of microRNA-96(miR-96)in cervical squamous cell carcinoma before and after neoadjuvant chemotherapy and to explore the effects of the treatment on expression of miR-96 and its relationship with clinical efficacy,chemosensitivity,and clinicopathologic features.Methods The expression of miR-96 was detected by real-time polymerase chain reaction in 28 patients with cervical squamous cell carcinoma(stage Ⅰb2-Ⅱa)before and after neoadjuvant chemotherapy.The correlations between the change of miR-96 and related clinicopathologic features of cervical cancer(age,pathologic pattern,histological grade,depths of interstitial infiltration,lymph node metastasis,and clinical stage)were further analyzed.Results The relative expression of miR-96 in cervical squamous cell carcinoma before treatment was(5.330±5.069)times higher than after the treatment with neoadjuvant chemotherapy(P=0.000).The relative change of the expressions of miR-96 in cervical cancer patients between pre-and post-neoadjuvant chemotherapy was closely related with clinicopathological features:it was significantly smaller in poorly-or moderately-differentiated patients(2.345±1.153)than in well-differentiated patients(9.941±5.359)(P=0.000),significantly larger in patients with the tumor invasion depth＜1/2 interstice(8.236±5.399)than in≥1/2 interstice(1.978±1.030)(P=0.000),and significantly smaller in patients with lymph node metastasis(1.848±0.959)than those without lymph node metastasis(7.942±5.348)(P=0.000).There was no significant difference in terms of age and clinical stages(Ⅰb2-Ⅱa)(P＞0.05). Conclusions Neoadjuvant chemotherapy can effectively reduce miR-96 expression in cervical squamous cell carcinoma tissues.It is therefore speculated that detection of the miR-96 expression before the chemotherapy for cervical cancer may be helpful to predict the therapeutic efficacy and sensitivity of the treatment.

Key words: cervical squamous cell carcinoma, microRNA-96, neoadjuvant chemotherapy, chemosensitivity

CLC Number:

R711.74

ZHOU Wen,GUAN Ting. Expressions of MicroRNA-96 Before and After Chemotherapy and Its Relationship with Chemosensitivity in Cervical Squamous Cell Carcinoma[J].Acta Academiae Medicinae Sinica, 2014, 36(2): 140-144.

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Expressions of MicroRNA-96 Before and After Chemotherapy and Its Relationship with Chemosensitivity in Cervical Squamous Cell Carcinoma

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