Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica ›› 2014, Vol. 36 ›› Issue (4): 351-356.doi: 10.3881/j.issn.1000-503X.2014.04.002

• Original article • Previous Articles     Next Articles

Effects of Bisphenol-A on Blastocyst Development and Implantation

YAN Pei-pei1,PAN Xiao-yan1,WANG Hong-he1,LI Zhi-xin1,WANG Xue-nan2,LAI Qin1,SONG Wen-jing1,ZHAO Hua-yi1,DOU Zhao-hua1   

  1. 1Department of Histology and Embryology,Jilin Medical College,Jilin 132013,China; 2Reproductive Medicine Center of the Affiliated Hospital of Jining Medical College,Jining,Shandong 272029,China
  • Received:2013-12-09 Online:2014-08-31 Published:2014-08-31
  • Contact: PAN Xiao-yan Tel:0432-64560019,
  • Supported by:
    Supported by the National Natural Sciences Foundation of China(30930092)and the Tianjin Health Bureau Technology Fund(20l2KY09)

Abstract: Objective To determine the effects of bisphenol-A (BPA) on blastocyst development and implantation. Methods According to completely randomized grouping method,90 pregnant mice were divided into 100, 300, and 600 mg/(kg·d)BPA groups and control group. BPA-treated pregnant mice were orally administered with BPA at concentrations of 100, 300 and 600 mg/(kg·d) from day 0.5 to day 3.5 of their pregnancy. Blastocyst implantation and development were studied. Results In the 300 mg/(kg·d) BPA group,the number of implantation sites and implantation rate were significantly decreased. In the 600 mg/(kg·d) group,no implantation sites were observed among pregnant mice and BPA inhibited embryo implantation. Blastocyst development on day 4 was examined,and findings showed that the development rate and total numbers of blastocysts in BPA treatment groups had no significant difference from the control group. However,BPA at 300 and 600 mg/(kg·d) significantly reduced blastocyst hatching rate and dramatically increased the number of blastocyst apoptotic cells when compared with those in the control group. Conclusion BPA at a high concentration damages the blastocyst development before implantation and inhibits embryo implantation.

Key words: bisphenol-A, blastocyst development, embryo implantation, apoptosis

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